Oxidative DNA Damage Enhancement by Vitamin C Among Narghile Smokers and Non-smokers

Nora Tariq Mohammed

Status: Completed

Conditions: Narghile

Treatments: Dietary Supplement: Vitamin C (Ascorbic Acid) regularly has been taken

Study type: Interventional

Funder types: Other

Identifiers

NCT07141823

UoD-ODNS-2025-01

Details and patient eligibility

About

This study aims to evaluate oxidative DNA damage among hookah smokers compared to non-smokers by measuring biomarkers of oxidative stress in blood samples. The objective is to determine the impact of hookah smoking on DNA integrity and overall oxidative status in adults.

Full description

Hookah smoking has become increasingly popular in many regions, yet its potential impact on oxidative stress and DNA integrity is not fully understood. This study aims to investigate the level of oxidative DNA damage among hookah smokers compared to non-smokers by analyzing biomarkers in blood samples. A case-control observational design will be used, involving two groups of adult participants: regular hookah smokers and age-matched non-smokers. Blood samples will be collected from all participants and analyzed for markers of oxidative stress, including 8-hydroxy-2'-deoxyguanosine (8-OHdG) and malondialdehyde (MDA), using validated biochemical methods. The findings are expected to provide insights into the biological consequences of hookah smoking and support the development of public health interventions.

Enrollment: 96 patients

Sex: All

Ages: 18 to 30 months old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Adults aged 18-30 years

Current narghile (hookah) smokers for at least 1 year

Willing to provide informed consent

Exclusion criteria

• Healthy participant

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Single Blind

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96 participants in 2 patient groups

Narghile nonsmokers vitamin C taking

Experimental group

Description:

this group of participant take vitamin C for one month, and they are healthy volunteers

Treatment:

Dietary Supplement: Vitamin C (Ascorbic Acid) regularly has been taken

Narghile smokers

Experimental group

Description:

this group take vitamin c supplent for one month period and are healthy.

Treatment:

Dietary Supplement: Vitamin C (Ascorbic Acid) regularly has been taken