Oxidative Stress and Mitochondrial TERT in Papillary Thyroid Cancer. (TEMPEST)

Istituto Auxologico Italiano

Status: Completed

Conditions: Papillary Thyroid Cancer

Study type: Observational

Funder types: Other

Identifiers

NCT05752669

05M902

Details and patient eligibility

About

Oxidative stress (OS) could be involved in the progression of papillary thyroid cancer (PTC). Indeed, thyroid differentiation genes are silenced by a mechanism controlled by NOX4-derived OS. On the other hand, TERT contributes to mitochondrial OS protection, which could increase the resistance of cancer cells to therapeutic agents. The investigators aim to address the role of OS and mitochondrial TERT in the progression and therapeutic resistance of PTC. OS and TERT subcellular localization will be investigated in 150 PTCs and correlated to the genetic and expression profile of the tumors and to the clinical and prognostic features of the patients. Mechanisms implicated in TERT mitochondrial migration and the contribution of mitochondrial TERT to tumor progression will be investigated in cancer cell lines and primary cell cultures. This study will allow to identify OS as a marker of therapeutic resistance in PTC and will open new opportunities for the development of novel treatments targeting ROS generation/TERT nuclear export.

Enrollment: 100 patients

Sex: All

Volunteers: No Healthy Volunteers

Inclusion criteria

• Patients with papillary thyroid cancer
• Patients whose tumor and contralateral healthy tissues can be collected for the analyses

Exclusion criteria

• patients who did not provide consent
• patients lost at follow-up
• tissues not adequate for the analyses