Panton Valentine Leucocidin (PVL)

Civil Hospices of Lyon

Status

Completed

Conditions

Staphylococcus Aureus Pneumonias

Treatments

Other: Serum and Blood samples

Study type

Interventional

Funder types

Other

Identifiers

NCT02798497

2010.625

Details and patient eligibility

About

Staphylococcus aureus expresses a variety of virulence factors, including Panton Valentine leukocidin (PVL), a cytotoxin. PVL is specifically associated with primary skin and soft-tissue infections and severe necrotizing pneumonia (Gillet et al. Lancet, 2002;359:753-9). PVL-positive S. aureus pneumonia is often preceded by influenza-like symptoms, and is mainly characterized by hemoptysis, pleural effusion, rapid onset of acute respiratory distress, leukopenia and a high fatality rate (65%) (Gillet et al. Lancet, 2002;359:753-9). Ten year after the first description of this disease and a number of controversies in the scientific literature, the question arise as to whether PVL remains an independent factor of severity in S.aureus pneumonia. In addition, numerous questions remain unanswered yet; these are:

(i) which factors, including treatment regimen, are associated with favourable outcome?,
(ii) what is the susceptibility toward antibiotics of strains associated with this disease ?
(iii) is there any genetic susceptibility of the host to explain both the rarity, and the explosive presentation of the disease ? To address the above questions a prospective observational study at the nationwide level will be set up. All French hospitals will be invited to describe the clinical features of all new cases of S. aureus community-acquired pneumonia with severity criteria, regardless PVL production. The study will include an investigation of a possible innate immune dysfunction in collaboration with the INSERM-U550 (Génétique Humaine des Maladies Infectieuses, Faculté Necker, Paris). Hence, in addition to collecting clinical and biological data from all pneumonia cases as well as all strains of S. aureus isolated, the patients with PVL-positive pneumonia will be sampled for immune genetic studies (ORFeome sequencing and functional studied)

Enrollment

234 patients

Sex

All

Ages

2 months to 100 years old

Volunteers

No Healthy Volunteers

Inclusion criteria

2-month-old patient at minimum and weighing at least 5 kg
Informed consent
Subjects affiliated (or beneficiary) to a national medical insurance
Presence of clinical, biological and radiological signs of pneumopathy to S. aureus whose clinical state justifies a hospitalization in a ICU or in a reanimation
Presence of PVL- positive S.aureus producer (for immunogenetic study)

Exclusion criteria

Patients infected by the HIV
Patients hospitalized for more than 48 hours at the time of the diagnosis of pneumonia,
Patients hospitalized during the previous three months excepted outpatients