Pathogens Responsible for Respiratory Infections Associated With Drowning in Seawater (APIR-NOY)

C

Centre Hospitalier Universitaire de Nice

Status

Enrolling

Conditions

Drowning

Treatments

Procedure: bronchoalveolar lavage

Study type

Interventional

Funder types

Other

Identifiers

NCT03498963
17-AOIP-03

Details and patient eligibility

About

Little data is currently available on the type of pathogen responsible for respiratory infections following drowning. Many environmental germs present in seawater are described as difficult to culture on standard media but are potentially pathogenic. Even using specific culture media nearly 90% of the bacteria present in the water remain non-cultivable. The use of 16S and 18S rDNA amplification followed by high throughput sequencing on respiratory samples could allow us to objectify these bacteria potentially involved in the physiopathological process secondary to drowning and thus improve their overall management. Carrying out a bronchoalveolar lavage (BAL) within 24 hours after admission to intensive care with analysis on standard and specific culture media of environmental germs, carrying out antibiograms, DNA extraction, amplification by universal 16 and 18S rDNA primers followed by high flow sequencing and phylogenetic analysis of pathogens found. Comparison of these results with the same analyses performed on seawater samples taken near the drowning site and during a new AML at 72h for patients still intubated and showing signs of pneumonia in order to determine if the pathogens detected at the entrance persist and are present in the environment. AML at the patient's entrance, on day 3 and environmental sampling in the drowning environment within 24 hours. Standard and specific bacteriological analyses with molecular biology techniques (amplification 16 and 18S rDNA) carried out at Pr RUIMY's laboratory (Nice University Hospital) sequencing on INRA's Toulouse genomics platform. Phylogenetic data capture and analysis at IRCAN (Bioinformatics, Croce Olivier). Patient follow-up until resuscitation discharge.

Enrollment

15 estimated patients

Sex

All

Ages

18+ years old

Volunteers

No Healthy Volunteers

Inclusion criteria

  • adult patient
  • hospitalized in intensive care for severe drowning in sea water (Spilzman stage 5 or 6) (4)
  • intubated within 24 hours of admission or before admission to intensive care
  • affiliated to social security

Exclusion criteria

patients under guardianship, trusteeship or detention

Trial design

Primary purpose

Basic Science

Allocation

N/A

Interventional model

Single Group Assignment

Masking

None (Open label)

15 participants in 1 patient group

bronchoalveolar lavage
Other group
Treatment:
Procedure: bronchoalveolar lavage

Trial contacts and locations

0

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Central trial contact

jean Dellamonica

Data sourced from clinicaltrials.gov

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