PENNVAX-B With or Without IL-12 or IL-15 as a DNA Vaccine for HIV Infection

University of Pennsylvania

Status and phase: Completed; Phase 1

Conditions: HIV Infection

Treatments: Biological: PENNVAX-B; Biological: PLACEBO; Biological: GENEVAX IL-12-4532; Biological: IL-15 adjuvant

Study type: Interventional

Funder types: Other

Identifiers

NCT00775424

807682

Details and patient eligibility

About

A phase Ib partially blinded pilot study to evaluate the safety and immunological effects of PENNVAX-B with or without co-administration of constructs containing DNA encoding for the expression of either IL-12 or IL-15.

Primary objectives

1. To determine the safety of HIV-1 DNA constructs (PENNVAX-B).
2. To determine the safety and optimal doses of the IL-12 and the IL-15 adjuvant constructs when given with PENNVAX-B.

Secondary objectives

1. To compare the various vaccine groups for their immunological responses to several HIV-1 antigens, utilizing the ELISPOT assay.
2. To analyze antibody responses to the vaccine antigens over time.
3. To measure CD8 cell proliferative responses to vaccine antigens over time.

Enrollment: 38 patients

Sex: All

Ages: 18 to 50 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

1. HIV-1 infection documented by any licensed ELISA test kit and confirmed by Western blot, HIV-1 culture, HIV-1 antigen, or plasma HIV-1 RNA, at any time before study entry.

2. Taking a stable HAART regimen for ≥3 months before the time of enrollment.

3. CD4-positive lymphocyte count ≥400 cells/µl on two occasions within 60 days of enrollment, performed at any certified flow laboratory.

4. HIV-1 < 75 copies/mL on two occasions within 60 days of enrollment, performed in a CLIA certified laboratory.

5. Laboratory values obtained within 30 days prior to study entry:

6. Hemoglobin > 9 g/dL (female subjects) and > 9.5 g/dL (male subjects)

7. Absolute neutrophil count > 1000 cells/μL

8. Platelet count > 75,000/μL

9. ALT, AST and alkaline phosphatase ≥ 2.5 x upper limit of normal range

10. Total bilirubin ≥ 2.5 x upper limit of the laboratory normal range

11. Serum creatinine ≥ upper limit of normal (ULN).

12. All women of reproductive potential (who have not reached menopause nor undergone hysterectomy, oophorectomy, or tubal ligation) must have a negative pregnancy test within 30 days of initiating study-specified medication(s) and at day 0 (enrollment).

    1. Women who are not of reproductive potential (have reached menopause or undergone hysterectomy, oophorectomy, or tubal ligation) or whose male partner has undergone successful vasectomy with resultant azoospermia or has azoospermia for any other reason, are eligible without requiring the use of contraception. Documentation of menopause, sterilization (hysterectomy, oophorectomy, tubal ligation, or vasectomy) and azoospermia by patient-reported history is acceptable.
    2. All subjects must not participate in a conception process (i.e. active attempt to become pregnant or to impregnate, sperm donation, in vitro fertilization), and if participating in sexual activity that could lead to pregnancy, the female study volunteer/male partner must use a form of contraception while receiving protocol-specified medication(s)/vaccinations and for one month after stopping the vaccinations.

13. Karnofsky performance score ≥ 90 within 30 days prior to study entry.

14. Men or women ≥18 years of age and less than 50.

15. Ability and willingness of subject or legal guardian/representative to give written informed consent.

Exclusion criteria

1. Any active or past AIDS-defining illness with the exception of minimal (less than 10 lesions) cutaneous Kaposi's sarcoma.
2. Subjects with a history of a CD4+ T-cell count ≤200/µl are not eligible.
3. Use of any known immunomodulatory therapy within 4 weeks prior to study entry including but not limited to drugs such as systemic corticosteroids, interferons, interleukins, thalidomide, granulocyte-macrophage colony-stimulating factor, IV gammaglobulin, or human growth hormone.
4. Any malignancy requiring systemic or local toxic chemotherapy. Local radiation will be allowed.
5. Pregnancy or breast-feeding.
6. Uncontrolled diabetes mellitus (fasting blood glucose > 126 mg/dL or random blood glucose levels > 200 mg/dL on at least two occasions within 6 months prior to study entry).
7. Major organ transplantation.
8. Active alcohol or substance abuse or psychiatric illness, which in the opinion of the investigator will interfere with adherence to study requirements.
9. Clinically significant neurological disorder occurring within 1 year prior to study entry that in the opinion of the principal investigator would affect the subject's study compliance or safety.
10. Use of systemic corticosteroids for ≥ 4 weeks within 3 months prior to study entry.
11. Presence of any chronic disease that in the opinion of the investigator might affect subject safety.
12. History of previous vaccination with an HIV-1 vaccine.
13. History or evidence of autoimmune disease, including, but not limited to thyroid autoimmune disease and idiopathic thrombocytopenic purpura.
14. Allergies to bupivacaine or similar anesthetic.

Trial design

Primary purpose: Treatment

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Quadruple Blind

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38 participants in 4 patient groups, including a placebo group

PENNVAX-B alone

Experimental group

Description:

PENNVAX-B alone

Treatment:

Biological: PENNVAX-B

PENNVAX-B+IL12

Experimental group

Description:

PENNVAX-B+IL12

Treatment:

Biological: GENEVAX IL-12-4532

Biological: PENNVAX-B

PENNVAX-B+IL15

Experimental group

Description:

PENNVAX-B+IL15

Treatment:

Biological: IL-15 adjuvant

Biological: PENNVAX-B

PLACEBO

Placebo Comparator group

Description:

PLACEBO

Treatment:

Biological: PLACEBO