Performance of 3 Sampling Methods for the Detection of SARS-CoV-2 (COVID-19) With Real-time Reverse Transcriptase PCR

Jessa Hospital

Status: Terminated

Conditions: Polymerase Chain Reaction; Diagnosis; Covid-19; SARS-CoV-2

Treatments: Other: samling of oropharynx and nasopharynx

Study type: Interventional

Funder types: Other

Identifiers

NCT04361448

20.43

Details and patient eligibility

About

The performance of 3 different sampling methods (2 nasopharyngeal swabs, 1 oropharyngeal swab) for the detection of SARS-CoV-2 with real-time reverse transcriptase polymerase chain reaction will be compared.

Full description

Thirty volunteers will be recruited from health care workers suspicious for Covid-19, who consult the ocupational physician for Covid-19 testing.

For each volunteer, 3 swabs will be taken:

• Flocked eSwab (Copan, Italy) of the oropharynx
• Flocked nasopharyngeal eSwab (Copan, Italy) of the nasopharynx
• Dry flocked nasopharyngeal Swab, placed in lysis buffer Each swab will be tested for the presence of SARS-CoV-2 with an in-house reverse-transcriptase real-time PCR (Egene).

Performance of each method will be calculated.

Enrollment: 30 patients

Sex: All

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• health care workers with symptoms suspicious for Covid-19

Exclusion criteria

• none

Trial design

Primary purpose: Diagnostic

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)

---

30 participants in 1 patient group

Health Care Workers with Covid-19 symptoms

Other group

Description:

3 samples (2 nasopharyngeasl swabs, 1 oropharyngeal swab) willl be taken from each volunteer

Treatment:

Other: samling of oropharynx and nasopharynx