Periodontal Regeneration Using Dental Pulp Stem Cells (DPSCs)

University of Turin

Status: Completed

Conditions: Periodontal Diseases

Treatments: Procedure: periodontal regeneration

Study type: Interventional

Funder types: Other

Identifiers

NCT03386877

UTurin stem cell RCT

Details and patient eligibility

About

The goal of this study is to evaluate if dental pulp stem cells (DPSCs) delivered into intrabony defects in a collagen scaffold would enhance the clinical and radiographic parameters of periodontal regeneration.

Full description

In this randomized controlled trial, 29 chronic periodontitis patients presenting one deep intrabony defect and requiring extraction of one vital tooth were consecutively enrolled. Defects were randomly assigned to test or control treatments which both consisted of the use of minimally invasive surgical technique. The dental pulp of the extracted tooth was mechanically dissociated to obtain micro-grafts rich in autologous DPSCs. Test sites (n=15) were filled with micro-grafts seeded onto collagen sponge, whereas control sites (n=14) with collagen sponge alone. Clinical and radiographic parameters were recorded at baseline, 6 and 12 months postoperatively.

Enrollment: 29 patients

Sex: All

Ages: 18+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• diagnosis of advanced chronic periodontitis
• FMPS<20%
• FMBS<20%
• infrabony defect >2mm
• Probing depth >5mm
• presence of 1 vital and intact tooth requiring an extraction as autologous source of DPSCs

Exclusion criteria

• smoking
• controindicazion for periodontal surgery
• systemic diseases affecting periodontal healing
• pregnancy an lactation
• fixed orthodontic appliance
• history of periodontal surgery at the experimental teeth
• prostethic restorations at the experimental teeth
• furcation defects

Trial design

Primary purpose: Treatment

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Triple Blind

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29 participants in 2 patient groups

Dental pulp stem cells

Experimental group

Description:

Test sites (n=15) Periodontal regeneration using micro-grafts of Dental pulp stem cells seeded onto collagen sponge

Treatment:

Procedure: periodontal regeneration

coagulum

Active Comparator group

Description:

control sites (n=14) Periodontal regeneration using coagulum and collagen sponge alone

Treatment:

Procedure: periodontal regeneration