Perioperative Evaluation of MMP-9-TIMP-1 System in Vascular Surgery Regarding Ischemic-reperfusion Injury

University of Pecs

Status: Completed

Conditions: Ischemia Reperfusion Injury

Treatments: Procedure: aorto-bifemoral bypass and aorta stentgraft

Study type: Observational

Funder types: Other

Identifiers

NCT03397732

4330

Details and patient eligibility

About

The MMP-9-TIMP-1 system has been implicated in many physiological and pathophysiological conditions including vascular surgery related ischemic-reperfusion injury. Our key aims were to establish the early perioperative time courses of the aforementioned system in aorto-bifemoral bypass and aorta stentgraft implantation procedures and to find correlation between the MMP-9-TIMP-1 system and the cross-clamp time. Patients were prospectively enrolled after Ethical Committee approval. Blood samples were taken at four different time points (T1-4): T1: right before surgery, T2: 60 min after the cross-clamp release, T3: first postoperative morning, T4: third postoperative morning. Plasma was isolated from heparin anticoagulated blood samples by low speed centrifugation at 4 °C, and stored at -80 °C until analyzed in a single batch at the end of the study. MMP- 9 and TIMP-1 were determined by the quantitative sandwich enzyme-linked immunosorbent assay (ELISA) techniques according to the manufacturer instructions (R&D Systems Inc., Minneapolis, Minnesota, USA). In comparison with standard curves, the concentrations of MMP-9 and TIMP-1 in plasma were determined spectrophotometrically (Multiskan Ascent microplate photometer, Type: 354, Thermo Electron Corporation, Waltham, Massachusetts, USA) by reading the absorbance at 450 nm. Plasma concentrations of MMP-9 and TIMP-1 were expressed as ng/ml.

Enrollment: 20 patients

Sex: All

Ages: 18+ years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Scheduled for elective procedure (aorto-bifemoral bypass or aorta stentgraft implantation) by vascular surgeons.
• Over age 18
• agree to participate

Exclusion criteria

• under age 18
• refuse to participate
• diagnosed malignant disease
• chronic steroid usage
• being on cytostatic therapy

Trial design

20 participants in 2 patient groups

Aorto-bifemoral bypass

Description:

Patients scheduled for elective aorto-bifemoral bypass surgery by vascular surgeons and consented to participate in the study.

Treatment:

Procedure: aorto-bifemoral bypass and aorta stentgraft

Aorta stentgraft

Description:

Patients scheduled for elective aorta stentgraft implantation by vascular surgeons and consented to participate in the study.

Treatment:

Procedure: aorto-bifemoral bypass and aorta stentgraft