Peripheral Blood Single Cell Sequencing Analysis of POD and CPSP in Elderly Patients After Total Knee Arthroplasty

Xuzhou Central Hospital (Xuzhou Fourth People's Hospital)

Status: Enrolling

Conditions: Chronic Postsurgical Pain; Postoperative Delirium

Study type: Observational

Funder types: Other

Identifiers

NCT05572307

XuzhouCH20221010

Details and patient eligibility

About

Single-celled sequencing for evaluating differences in gene expression patterns in different cell types of the dynamics of a means of this research as a starting point, to study the postoperative delirium and chronic pain at the cellular level changes the contents of a cell, reveal its occurrence and development of the role of gene regulation, find targets and biomarkers, and to provide new ideas for its pathogenesis, To provide theoretical basis support for its prevention, clinical diagnosis and treatment.

Full description

The aim of this study was to investigate leukocyte heterogeneity in patients undergoing total knee arthroplasty with POD, paired non-POD patients, and cognitively normal healthy subjects by single cell sequencing, and to investigate leukocyte heterogeneity in patients with chronic pain and patients with pain relief after surgery through postoperative pain follow-up. Moreover, multi-omics single cell technology is used to analyze various cellular contents in a single cell simultaneously, which can capture molecular footprints with higher resolution. Comprehensive analysis of multiple omics datasets reveals the relationships between cellular patterns, establishes complex regulatory networks, and provides a holistic view of cellular state. From a single cell level analysis of POD and occurred in patients with chronic pain in patients with the characteristics of the white blood cells, looking for the peripheral immune system in the pathogenesis of the POD and the potential role of chronic pain occurs, may provide POD pathogenesis and chronic pain occurred an important visual Angle, may also help to provide POD biomarkers and therapeutic targets, To provide a possible solution for the prevention and treatment of postoperative delirium and postoperative chronic pain.

Enrollment: 10 estimated patients

Sex: All

Ages: 65 to 80 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Age ≥65 years old, regardless of gender.
• total knee arthroplasty for the first time.
• The ASA classification is I-Ⅲ.

Exclusion criteria

• severe neurological or psychiatric disorders
• drug and alcohol abuse
• patients on antidepressants
• failure and decompensation of vital organs
• chronic pain from previous surgery or trauma

Trial design

10 participants in 4 patient groups

POD group

Description:

According to the occurrence of postoperative delirium, the patients were divided into POD group and non-POD group

non-POD group

Description:

According to the occurrence of postoperative delirium, the patients were divided into POD group and non-POD group

CPSP group

Description:

Patients were divided into CPSP group and non-CPSP group according to the occurrence of postoperative chronic pain

non-CSP group

Description:

Patients were divided into CPSP group and non-CPSP group according to the occurrence of postoperative chronic pain