PGTai Results for Embryos Cultured in Sequential Versus Single Step Media

Ganin Fertility Center

Status: Unknown

Conditions: Mosaicism; Aneuploidy Rate; Infertility

Treatments: Other: Sequential media; Other: Single step media

Study type: Interventional

Funder types: Other

Identifiers

NCT04485910

MH0004

Details and patient eligibility

About

A prospective cohort study undergoing ICSI at a private fertility center in Egypt

Full description

Purpose:

To evaluate PGTai results for embryos cultured in sequential versus single step media.

Method:

Injected oocytes will divided between Continuous Single Culture-NX media (CSC- NX) with 10% SSS the first group (Irvine, USA) and the other group will be cultured in sequential cleavage and blastocyst media with 10% SPS (SAGE, Denmark).

All blastocytes with 4BB quality and better according to Gardner's criteria 1999 will go through PGTA procedures with PGTai confirmation.

Enrollment: 200 estimated patients

Sex: All

Ages: 18 to 37 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Female age ≤37 years with ≥ 8 mature oocytes
• Male partner had normal semen parameters according to WHO 2010
• PGT-A was done at the blastocyst stage

Exclusion criteria

• sperm or oocyte donor
• severe male factors according to WHO 2010

Trial design

Primary purpose: Other

Allocation: Non-Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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200 participants in 2 patient groups

Single step media

Active Comparator group

Description:

Embryos cultured in single step media

Treatment:

Other: Single step media

Sequential media

Active Comparator group

Description:

Embryos cultured in sequential media

Treatment:

Other: Sequential media