Pharmacokinetic Interactions of Metamizole (Dipyrone) in Healthy Subjects

University Hospital Basel

Status and phase

Completed

Phase 1

Conditions

Drug-Drug Interaction

Induction

Inhibition

Treatments

Drug: Metamizole

Study type

Interventional

Funder types

Other

Identifiers

NCT03990129

2018-00753

Details and patient eligibility

About

Investigators conducted a single center, two-phased, open, controlled pharmacokinetic study to investigate the drug-drug interaction potential of metamizole. For this reason, healthy male volunteers were screened.

Enrolled participants were phenotyped on day 1 using the Basel Cocktail (phenotyping cocktail containing specific substrates for CYP1A2, CYP2B6, CYP2C9, CYP2C19, CYP2D6, CYP3A4). After, they received metamizole treatment for 8 days (3 grams per day). On the 8th day (day 9), they were phenotyped again with the Basel Cocktail and the respective phenotypes (d1 vs. d9) were compared.

Full description

12 healthy male subjects meeting the inclusion and exclusion criteria were enrolled. On day 1, they ingested a capsule containing the Basel Cocktail (1A2: caffeine, 2B6: efavirenz, 2C9: flurbiprofen, 2C19: omeprazole, 2D6: metoprolol, 3A4: midazolam) in fastened state. Blood samples were withdrawn over 24 hours and the AUC ratios between metabolite and parent were calculated to determine the phenotype.

Probands began metamizole treatment (3000 mg/day) at the day of the 24h measurement. After 3-4 day, probands were returning to the facility to ensure safety during the metamizole treatment, blood cell count and metamizole metabolites were measured. After the 7 days of treatment, probands were exposed again to the Basel Cocktail in a fastened state. Probands were still exposed to metamizole to ensure potential inhibition. 24 hours plasma samples were withdrawn, area under the curve (AUC) ratios were calculated and compared to the basal state. After an end of study visit on the day of the 24h blood sample (following the second study day), probands were discharged.

Enrollment

12 patients

Sex

Male

Ages

18 to 45 years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

Healthy subjects aged between 18 and 45 years (inclusive) at screening
BMI between 18 and 28 kg/m2 (inclusive) and bodyweight at least 50 kg at screening
systolic blood pressure: 100-140 mmHg, diastolic blood pressure: 60-90 mmHg and heart rate: 45-90 bpm (inclusive), measured on the leading arm, in the supine position at screening
No clinically significant findings on the physical examination at screening
Signed informed consent prior to any study-mandated procedure
Hematology and clinical chemistry results not deviating from the normal range to clinically relevant extent at screening
Ability to communicate well with the investigator to understand and comply with the requirements of the study

Exclusion criteria

Smoking >5 cigarettes per day
History or clinical evidence of alcoholism or drug abuse within the 3- year period prior to screening
Loss of ≥250 ml of blood within 3 months prior to screening. Treatment with an investigational drug within 30 days prior to screening
Previous systemic treatment with any prescribed or over the counter medication (including herbal medicines such as St John's Wort) within 2 weeks prior to the intended start of the study
Inability to stop consumption inducing food products (e.g. grapefruit juice) 72 h before start of the study
Excessive caffeine consumption (>8 cups of coffee/ 8l coca cola per day)
Legal incapacity or limited legal capacity at screening
Positive results from urine drug screen at screening
History or clinical evidence of any disease (e.g. gastrointestinal disease: Morbus Crohn, Colitis Ulcerosa, anamnestic gastrointestinal bleeding) and/or existence of any surgical or medical condition, which might interfere with the absorption, distribution, metabolism or excretion of the study drugs, or which might increase the risk for toxicity
Known hypersensitivity to Metamizole (Novalgin®) or excipients of the drug formulation
Any circumstances or conditions, which, in the opinion of the investigator, may affect full participation in the study or compliance with the protocol.