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Phase 1 Study to Evaluate the Safety and Tolerability of Intravenously Administered PYC-003

P

PYC Therapeutics

Status and phase

Enrolling
Phase 1

Conditions

Autosomal Dominant Polycystic Kidney Disease (ADPKD)

Treatments

Drug: PYC-003

Study type

Interventional

Funder types

Industry

Identifiers

NCT06714006
PYC-003-CL-001

Details and patient eligibility

About

This is a Phase 1, First-in-Human study to evaluate the safety and tolerability of PYC-003 in healthy adult participants and adult participants with confirmed PKD1 mutation-associated ADPKD. There are 2 parts in this study, i.e. Part A and Part B.

Full description

Part A (SAD - Healthy) will be conducted as a randomized, double-blind, placebo-controlled, SAD study to assess the safety, tolerability, PK, PD, and immunogenicity of PYC-003 in healthy adult participants. The anticipated number of participants across 3 Part A (SAD - Healthy) cohorts is approximately 24 participants. On Day 1, each participant will receive the investigational product (IP; ie, PYC-003 or placebo), as a single intravenous (IV) infusion. All Part A (SAD - Healthy) cohorts will first dose 2 sentinel participants in a blinded manner on Day 1.

Part B (SAD - ADPKD) will be conducted as an open-label SAD study to assess the safety, tolerability, PK, PD, and immunogenicity of PYC-003 in adult participants with confirmed PKD1mutation-associated ADPKD. The anticipated number of participants across 3 Part B (SAD - ADPKD) cohorts is approximately 18 participants. On Day 1, each participant will receive PYC-003 as a single IV infusion.

Enrollment

56 estimated patients

Sex

All

Ages

18 to 60 years old

Volunteers

Accepts Healthy Volunteers

Inclusion and exclusion criteria

Inclusion Criteria (Part A):

  • Male or female aged 18 to 60 years (inclusive) at the time of informed consent.
  • At the discretion of the PI or designee, in good general health, with no significant medical history, and have no clinically significant abnormalities on physical examination at Screening and/or before the first administration of IP
  • BMI between ≥ 18.0 and ≤ 32.0 kg/m2 and weight ≥ 50 kg
  • Nonsmoker and must not have used any tobacco products within 2 months prior to Screening.
  • Clinical laboratory values within normal range as specified by the testing laboratory, unless deemed not clinically significant by the PI or designee
  • Estimated glomerular filtration rate (eGFR) ≥ 80 mL/min/1.73 m2 via the Chronic Kidney Disease Epidemiology Collaboration (CKD EPI) 2021 calculation
  • Women of childbearing potential (WOCBP) must agree to use an acceptable, highly effective, double barrier method of contraception from the start of Screening until study completion.
  • Males must be surgically sterile or, if engaged in sexual relations with a WOCBP, must agree to use an acceptable, highly effective, double barrier method of contraception from the start of Screening until study completion
  • Females must agree not to donate ova from the first administration of IP until 30 days following study completion
  • Males must agree not donate sperm from the first administration of IP until 90 days following study completion
  • Able and willing to attend the necessary visits to the study site
  • Able and willing to adhere to caffeine, alcohol, and nicotine-containing product restrictions
  • Able and willing to provide written informed consent after the nature of the study has been explained and prior to the commencement of any study procedures

Inclusion Criteria (Part B):

  • Male or female aged 18 to 60 years (inclusive) at the time of informed consent.

  • ADPKD diagnosis based on Ravine Pei diagnosis criteria (Pei et al. 2009):

    1. Presence of ≥ 3 (unilateral or bilateral) renal cysts for participants aged ≤ 39 years.
    2. Presence of ≥ 2 renal cysts in each kidney for participants aged 40 to 55 years (inclusive).
  • ADPKD diagnosis as confirmed by the presence of genetic mutations associated with ADPKD (per genotyping by a National Association of Testing Authorities [NATA] accredited or equivalent diagnostic laboratory) including, but not limited to, the presence of PKD1 mutation.

Note: Where genotyping is not included the medical history for a participant, genotyping may be completed at Screening.

  • Class 1C, 1D, or 1E per Mayo Imaging Classification System for Predicting Kidney Outcomes in ADPKD (Irazabal et al. 2015) (based upon prior magnetic resonance imaging [MRI] or computed tomography [CT] scan obtained > 6 months prior to Screening, or MRI obtained during Screening).

  • BMI between ≥ 18.0 and ≤ 32.0 kg/m2 and weight ≥ 50 kg.

  • Nonsmoker and must not have used any tobacco products within 2 months prior to Screening.

  • Estimated glomerular filtration rate (eGFR) ≥ 30 and < 90 mL/min/1.73 m2 via the CKD EPI 2021 calculation

  • Hematology and serum chemistry results at Screening that meet the following criteria:

    1. Platelets > 150 × 109/L.
    2. Total white blood cell count > 3.0 × 109/L.
    3. Absolute neutrophil count > 1.5 × 109/L.
    4. Hemoglobin > 12 g/dL for females and > 13.5 g/dL for males.
    5. Total and direct bilirubin < 1.5 × ULN, unless elevated bilirubin is associated with a known benign condition (eg, Gilbert's syndrome).
    6. Alanine aminotransferase (ALT) < 1.5 × ULN.
    7. Aspartate aminotransferase (AST) < 1.5 × ULN.
    8. Alkaline phosphatase (ALP) < 1.5 × ULN.
    9. Gamma-glutamyl transferase < 2 × ULN. Note: Screening laboratory testing may be repeated once at the discretion of the PI or designee to confirm out of range (exclusionary) results.
  • WOCBP (see definition in Section 5.3) must agree to use an acceptable, highly effective, double barrier method of contraception from the start of Screening until study completion. Acceptable methods of contraception are defined in Section 5.4.4.1.

Note: Females with same-sex partners (abstinence from penile-vaginal intercourse) or who are abstinent from heterosexual intercourse are not required to use contraception when this is their preferred and usual lifestyle.

  • Males must be surgically sterile (vasectomized for at least 6 months prior to first administration of IP) or, if engaged in sexual relations with a WOCBP, must agree to use an acceptable, highly effective, double barrier method of contraception from the start of Screening until study completion. Acceptable methods of contraception are defined in Section 5.4.4.1.
  • Females must agree not to donate ova from the first administration of IP until 30 days following study completion.
  • Males must agree not donate sperm from the first administration of IP until 90 days following study completion.
  • Able and willing to attend the necessary visits to the study site.
  • Able and willing to adhere to caffeine, alcohol, and nicotine-containing product restrictions
  • Able and willing to provide written informed consent after the nature of the study has been explained and prior to the commencement of any study procedures.

Exclusion Criteria (Part A):

  • Females who are pregnant, breastfeeding, or plan to become pregnant during the course of the study

  • Underlying physical or psychological medical condition that, in the opinion of the PI or designee, would make the participant unlikely to comply with the protocol or complete the study per protocol

  • Has only 1 kidney or has received a kidney transplant

  • Blood donation or has significant blood loss (> 500 mL) within 30 days prior to the first administration of IP

  • Plasma donation within 7 days prior to the first administration of IP

  • Fever (body temperature >38°C) or symptomatic viral or bacterial infection within 2 weeks prior to Screening

  • Infections requiring parenteral antibiotics within 6 months prior to Screening

  • Positive test for hepatitis C antibody (HCV), hepatitis B surface antigen (HBsAg), HIV antibody.

  • History of life threatening infection (eg, meningitis).

  • Vaccination with a live vaccine within 4 weeks prior to the first administration of IP.

  • Poor venous access.

  • History of severe allergic or anaphylactic reactions, or sensitivity to the IP or its constituents.

  • History of malignancy, except for non-melanoma skin cancer, excised more than 2 years ago and cervical intraepithelial neoplasia that has been successfully cured more than 5 years prior to Screening.

  • Abnormal ECG findings at Screening, Day -1, or predose that are considered by the PI or designee to be clinically significant.

  • History or presence of a condition associated with significant immunosuppression

  • Exposure to any drugs that cause significant immunosuppression (including experimental therapies as part of a clinical study) within 4 months or 5 half lives (whichever is longer), prior to Screening.

  • ALP, AST, and ALT >1.5 × ULN at Screening or Day -1 Note: Repeat testing (ie, 1 repeat per parameter) at Screening is acceptable for out-of-range values following approval by the PI or designee.

  • History of borderline to low blood magnesium and potassium levels and/or Screening or Day -1 blood magnesium and potassium levels <0.7mmol/L and potassium levels < 4.0 mmol/L Note: Repeat testing (ie, 1 repeat per parameter) at Screening is acceptable for out of range values following approval by the PI or designee permitted.

  • Active infection of the urinary tract (ie, kidney, bladder).

  • Positive toxicology screening panel (urine test including qualitative identification of barbiturates, tetrahydrocannabinol [THC], amphetamines, benzodiazepines, opiates, and cocaine), or alcohol breath test.

  • History of substance abuse or dependency or history of recreational IV drug use over the last 5 years (by self-declaration).

  • Regular alcohol consumption defined as > 14 standard drinks per week for females and > 21 standard drinks for males (where 1 standard drink = 375 mL of mid-strength beer [3.5% alcohol/volume], 100 mL wine [13.5% alcohol/volume] or 30 mL of spirits [40% alcohol/volume]) or > 4 standard drinks on any single day.

  • Unwilling to abstain from alcohol for 48 hours prior to admission to the study site and for 48 hours prior to any follow-up visits.

  • Use of any investigational medical device or investigational drug within 30 days or 5 half lives of the investigational drug (whichever is longer) prior to the first administration of IP.

  • Use of (or anticipated use of) the following:

    1. Any prescription drugs (other than hormonal contraception; oral contraceptive pills, long-acting implantable hormones, injectable hormones, a vaginal ring, or an intrauterine device [IUD]) within 14 days prior to the first administration of IP and during the course of the study without prior approval of the PI and MM.
    2. Any over-the-counter medication, herbal remedies, supplements or vitamins within 7 days prior to the first administration of IP and during the course of the study without prior approval of the PI and MM.
  • Unwilling to refrain from strenuous exercise (including weightlifting) for 48 hours prior to admission to the study site and for 48 hours prior to any follow-up visits.

  • Anything that the PI considers would jeopardize the safety of the participant, prevent complete participation in the study, or compromise interpretation of study data.

Exclusion Criteria (Part B):

  1. Females who are pregnant or plan to become pregnant during the course of the study.

  2. Presence of potentially confounding genetic mutations (per genotyping by a NATA accredited or equivalent diagnostic laboratory) including, but not limited to, the presence of PKD2, HFN1B, GANAB, IFT140, and/or DNAJB 11 mutations.

  3. Use of (or anticipated use of) Tolvaptan and/or metformin administration within 30 days prior to the first administration of IP until study completion.

  4. Underlying physical or psychological medical condition that, in the opinion of the PI or designee, would make the participant unlikely to comply with the protocol or complete the study per protocol.

  5. Any renal or systemic pathology other than ADPKD or any other condition or prior therapy that in the opinion of the PI or designee would make the participant unsuitable for this study.

  6. Has only 1 kidney or has received a kidney transplant.

  7. Blood donation or had significant blood loss (> 500 mL) within 30 days prior to the first administration of IP.

  8. Plasma donation within 7 days prior to the first administration of IP.

  9. Has received (or is anticipated to receive) cell therapy, gene therapy, or RNA therapy for any renal condition.

  10. Fever (body temperature >38°C) or symptomatic viral or bacterial infection within 2 weeks prior to Screening.

  11. Infections requiring parenteral antibiotics within 6 months prior to Screening.

  12. Positive test for hepatitis C antibody (HCV), hepatitis B surface antigen (HBsAg), HIV antibody.

  13. History of life-threatening infection (eg, meningitis).

  14. Vaccination with a live vaccine within 4 weeks prior to the first administration of IP.

  15. Poor venous access.

  16. History of severe allergic or anaphylactic reactions, or sensitivity to the IP or its constituents.

  17. History of malignancy, except for non-melanoma skin cancer excised more than 1 year prior to Screening and cervical intraepithelial neoplasia that has been successfully cured more than 2 years prior to Screening.

  18. Abnormal ECG findings at Screening, Day -1, or predose that are considered by the PI or designee to be clinically significant.

  19. Abnormal vital signs findings at Screening that are considered by the PI or designee to be clinically significant.

    Note: A hypertensive participant is eligible if on a stable antihypertensive regimen for ≥ 28 days prior to first administration of IP and the blood pressure adequately controlled (per PI discretion) prior to first administration of IP.

  20. History or presence of a condition associated with significant immunosuppression.

  21. Exposure to any drugs that cause significant immunosuppression (including experimental therapies as part of a clinical study) within 4 months or 5 half-lives (whichever is longer), prior to Screening.

  22. History of borderline to low blood magnesium and potassium levels and/or Screening or Day -1 blood magnesium level < 0.7 mmol/L and potassium levels < 4.0 mmol/L.

    Note: Repeat testing (ie, 1 repeat per parameter) at Screening or Day -1 is permitted for out-of-range values following approval by the PI or designee.

  23. Proteinuria > 500 milligrams per 24 hours.

  24. Hematuria, proteinuria, and/or urinary abnormalities at Screening deemed by the PI or designee to be of moderate or higher severity.

  25. Renal complications (eg, cyst rupture or cyst infections) within 6 weeks prior to first administration of IP.

  26. Active infection of the urinary tract (ie, kidney, bladder).

  27. Positive toxicology screening panel (urine test including qualitative identification of amphetamines, barbiturates, benzodiazepines, cocaine, methamphetamine, methadone, opiates, phencyclidine, tetrahydrocannabinol [THC], tricyclic antidepressants), or alcohol breath test.

    Note: Repeat testing (ie, 1 repeat) at Screening or Day -1 is permitted where a participant has used a prescribed medication following approval by the PI or designee.

  28. History of substance abuse or dependency or history of recreational IV drug use over the last 5 years (by self-declaration).

  29. Regular alcohol consumption defined as > 14 standard drinks per week for females and > 21 standard drinks for males (where 1 standard drink = 375 mL of mid-strength beer [3.5% alcohol/volume], 100 mL wine [13.5% alcohol/volume] or 30 mL of spirits [40% alcohol/volume]) or > 4 standard drinks on any single day.

  30. Unwilling to abstain from alcohol for 48 hours prior to admission to the study site and for 48 hours prior to any follow-up visits.

  31. Use of any investigational medical device or investigational drug within 30 days or 5 half-lives of the investigational drug (whichever is longer) prior to the first administration of IP.

  32. Unwilling to refrain from strenuous exercise (including weightlifting) for 48 hours prior to admission to the study site and for 48 hours prior to any follow-up visits.

  33. Anything that the PI considers would jeopardize the safety of the participant, prevent complete participation in the study, or compromise interpretation of study data.

Trial design

Primary purpose

Treatment

Allocation

Randomized

Interventional model

Parallel Assignment

Masking

Triple Blind

56 participants in 2 patient groups, including a placebo group

Part A (SAD - Healthy)
Placebo Comparator group
Description:
Part A will be conducted as a randomized, double-blend, placebo-controlled, Single Ascending Dose Study to assess the safety, tolerability, Pharmacokinetic, Pharmacodynamic, and immunogenicity of PYC-003 in healthy adult participants
Treatment:
Drug: PYC-003
Part B (SAD - ADPKD)
Active Comparator group
Description:
Part B will be conducted as an open-label Single Ascending Dose study to assess the safety, tolerability, Pharmacokinetic, Pharmacodynamic, and immunogenicity of PYC-003 in adult participants with confirmed PKD1 mutation-associated ADPKD.
Treatment:
Drug: PYC-003

Trial contacts and locations

5

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Central trial contact

Sreenivasu Mudumba Chief Research & Development Officer, PhD

Data sourced from clinicaltrials.gov

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