Plasma Levels of Danger-Associated Molecular Patterns in Young Children After Cardiac Surgery Under Cardiopulmonary Bypass (IMMUNOPED2)

Nantes University Hospital (NUH)

Status: Completed

Conditions: Cardiac Surgical Procedure

Treatments: Diagnostic Test: Blood drop sampling

Study type: Observational

Funder types: Other

Identifiers

NCT04423523

RC19_0402

Details and patient eligibility

About

The investigators have previously reported that cardiac surgery with cardiopulmonary bypass (CPB) in young infants induced a drastic reduction in mHLA-DR (Human Leucocyte Antigen) expression, which represents one of innate immune mediator. Danger-Associated Molecular Patterns (DAMPs) can elicit immune response and may subsequently induce an immune-suppressed state. We hypothesize that analyzing the proteomic and the whole blood transcriptomic response after cardiac surgery would provide new insights into the pathophysiological mechanisms of CPB-related inflammatory response.

Full description

Plasma levels of heat shock protein and inflammatory-related proteins will be measured at four time points (prior to the onset of surgery, Hour 6 postoperative, day 1 and day 3 after surgery). Whole blood transcriptomic analysis will be performed before and after surgery. The immune suppression induced by CPB will be assessed by in vitro experiments.

Enrollment: 88 patients

Sex: All

Ages: Under 3 months old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Patient admitted to pediatric intensive care at Nantes University Hospital after cardiac surgery
• Age ≤ 3 months corrected age.
• Control group: patients less than 3 months old following cardiac surgery without CPB or digestive surgery with a central venous catheter.

Exclusion criteria

• Futility of care with cessation of blood tests as part of a limitation of active therapies;
• Absence of parents for information
• Refusal of parents after information (Lack of parental consent)
• Blood volume collected for medical purposes incompatible with the needs of the research.

Trial design

88 participants in 2 patient groups

Study population

Description:

The study population must correspond to the research inclusion criteria: Patient admitted to pediatric intensive care at Nantes University Hospital after cardiac surgery on bypass grafts

Treatment:

Diagnostic Test: Blood drop sampling

Control group

Description:

Infants under 3 months of age undergoing heavy digestive surgery and having a central venous catheter to facilitate access to blood samples.

Treatment:

Diagnostic Test: Blood drop sampling