Platelet-associated Inflammation in Severe Sepsis (PlatISSep)

Centre Hospitalier Universitaire de Saint Etienne

Status

Completed

Conditions

Severe Sepsis

Treatments

Other: Blood samples

Study type

Observational

Funder types

Other

Identifiers

NCT03029039

ID-RCB (Other Identifier)

1608110

Details and patient eligibility

About

Sepsis represents a serious public health issue characterized by a complex inflammatory response. In addition to their hemostatic role, platelets display inflammatory functions by secreting a variety of immunomodulatory factors and interacting with circulating immune cells. The investigators postulate that, in severe sepsis, platelets become activated and release amounts of different soluble inflammatory molecules that contribute to sepsis-associated inflammation. First, the investigators propose to assess whether severe sepsis impairs the ability of platelets to release soluble CD40L (sCD40L), an powerful platelet-derived immunomodulatory molecule, in ICU patients with S. aureus documented infection, ICU patients with documented infection involving other bacterial species, compared to ICU patients with inflammation of noninfectious origin and healthy blood donors. Then, the investigators wish to assess whether the bacterial species affects the release of platelet sCD40L and by an extensive screening of platelet soluble factors, the investigators propose to set up profiles of inflammatory molecules associated with the type of infection. Finally, the investigators will analyze platelets' activation state and their association with circulating immune, according to the type of infection. Therefore, this project is expected to assess to which extent the platelet inflammatory function is super-activated in severe sepsis and to identify new platelet-related biomarkers of sepsis.

Enrollment

127 patients

Sex

All

Ages

18+ years old

Volunteers

Accepts Healthy Volunteers

Inclusion and exclusion criteria

Inclusion criteria

Common inclusion criteria for ICU patients : Signed informed consent, Patient affiliated or entitled to a social security, aged over 18 years,

Criteria for blood donor voluntary : to weigh more than 50 kg
Criteria for severe sepsis group : Sepsis with failure of at least one organ, severe sepsis for less than 72 hours, sepsis with bacteria S. aureus, S. pneumoniae or E. coli.
Criteria for uninfectious inflammatory syndrome group : patients operate since less than 24 hours of hip or knee surgery, Absence of systemic infection

Exclusion Criteria

failure to participate at the study
Patients with a aspirin treatment has continued throughout severe sepsis
Patients with an appropriate antibiotic therapy for more than 72 hours
Patients with a platelet transfusion in the week before inclusion or 72 hours after a surgical gesture or the occurrence of sepsis
Patients with the platelet account is less than 30 000 per cubic millimeter the day of the sampling
Patients with a severe sepsis who had a surgical gesture previous week the inclusion
All clinical sequelae or biological at the selection
pregnant woman
Patients with a treatment by platelet aggregation has continued throughout severe sepsis