Polar Body Removal Before ICSI for Oocyte Activation in Previous Fertilization Failure Cases (PBRBICSI)

Benha University

Status: Completed

Conditions: Fertilization

Treatments: Procedure: Classic ICSI; Procedure: polar body removal using zona drilling by laser then suction by injecting pipette

Study type: Interventional

Funder types: Other

Identifiers

NCT02913781

Hawaa-2

Details and patient eligibility

About

Cases which undergone ICSI before with failure of fertilization will do another ICSI cycle with removal of the polar body just before the ICSI procedure and sperm injection at the site of polar body removal. This study group will be evaluated with another group with the classic ICSI procedure

Full description

Patients with previous fertilization failure will be divided into 2 groups:

Group 1 (control): Will undergo classic ICSI procedure in half the MII oocyte of the same case.

Group 2 (study group): The other half of the MII oocyte of the same case will undergo:

1. Polar body removal.
2. ICSI procedure with injection of the spermatozoon in the same zona opening (done by laser for polar body removal).

Results: Will be collected with evaluation of number of 2PN zygotes, Day 1, Day 2, Day 3, Day 5 embryos in all the cases.

Enrollment: 40 patients

Sex: Female

Ages: 20 to 40 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• previous fertilization failure
• previous fertilization arrest

Exclusion criteria

• NO MII oocyte
• Refusal of the procedure by the couple

Trial design

Primary purpose: Treatment

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)

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40 participants in 1 patient group

polar body removal

Experimental group

Description:

polar body removal by zona drilling with laser then suction by injecting pipette then ICSI procedure is done

Treatment:

Procedure: Classic ICSI

Procedure: polar body removal using zona drilling by laser then suction by injecting pipette