Polymorphisms in ENAM, AMBN, and KLK4 Contribute to DCS.

Amina Fouad Farag

Status: Completed

Conditions: Dental Caries Secondary to Polymorphisms; Polymorphisms in Enamel Encoding Genes

Treatments: Genetic: DNA collation and genotyping

Study type: Observational

Funder types: Other

Identifiers

NCT06343766

RECO6U/24-2022

Details and patient eligibility

About

A Cross-Sectional Study aims to shed light on potential genetic determinants influencing dental caries susceptibility within this demographic and significantly understanding of genetic factors associated with dental caries, particularly within the context of the Egyptian population, providing valuable insights into the genetic aspects of oral health. The research methodology involved a comprehensive examination of these polymorphic loci ENAM, AMBN, TUFT1 and KLK4 and their prevalence, employing rigorous statistical analyses to establish potential correlations between these genetic variants and susceptibility to dental caries.

Full description

The research investigates the distributional discrepancies of specific polymorphic loci ENAM rs3796703, AMBN rs4694075, TUFT1 rs13236243, and KLK4 rs2242670-within the Egyptian population as correlatives and causatives of dental caries susceptibility through the genotyping of enamelin (ENAM rs3796703), ameloblastin (AMBN rs4694075), tuftelin 1 (TUFT1 rs78802584), and kallikrein 4 (KLK4 rs2242670) in 132 adults (males = 74, females = 58) and 72 controls (males = 40, females = 32) referred from various Egyptian hospitals. For each participant, the number of decayed, missing, and filled teeth was charted, and the presence of biofilm/gingivitis/fluorosis was assessed. Bitewing radiographs were taken to detect interproximal caries. In addition, statistical analysis was conducted using Chi-square test, odds ratios, and corresponding P-values.

Enrollment: 204 patients

Sex: All

Ages: 15 to 22 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Presence of dental caries or decay in form of discoloration, cavities, or visible enamel softening suggestive of potential caries (Caries-susceptible group)
• Caries-free patients as control group (Caries-resistant group)

Exclusion criteria

• The presence of white spots or fluorosis, indicating enamel defects or demineralization, required exclusion from the study.

Trial design

204 participants in 2 patient groups

Experimental group

Description:

Caries-susceptible group

Treatment:

Genetic: DNA collation and genotyping

Control group

Description:

Caries-resistant group

Treatment:

Genetic: DNA collation and genotyping