Postprandial IHL and IMCL Measured With Proton Magnetic Resonance Spectroscopy

Maastricht University Medical Centre (MUMC)

Status: Completed

Conditions: Postprandial IHL and IMCL Dynamics

Treatments: Dietary Supplement: HF breakfast with protein; Dietary Supplement: High fat breakfast

Study type: Interventional

Funder types: Other

Identifiers

NCT01709643

METC 12-3-025

Details and patient eligibility

About

The major research objective is to assess the potential of Proton Magnetic Resonance Spectroscopy to follow Intrahepatic lipid and Intramyocellular lipid dynamics after a single meal. Furthermore we aim to investigate whether the addition of protein to a high fat breakfast results in a reduced postprandial accumulation of lipids when compared to a high fat breakfast alone.

Full description

Fat accumulation in non-adipose tissue, such as liver and muscle, is associated with reduced insulin sensitivity and cardiovascular disease. Little is known about the dietary determinants and the time course of this ectopic fat accumulation. Therefore the general aim of this project is to develop and validate methodology to follow postprandial hepatic and muscular lipid retention. Proton Magnetic Resonance Spectroscopy (1H-MRS) is a well-established tool for noninvasive quantification of intrahepatic lipids (IHL) and intramyocellular lipids (IMCL). Previous studies have suggested a relatively rapid regulation of IHL and IMCL levels, indicated by changes in these total lipid pools after high fat diets of 3 or 4 days. Although IHL and IMCL levels seem to change rapidly, data on IHL and IMCL levels after a single meal are lacking.

Enrollment: 9 patients

Sex: All

Ages: 18 to 40 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Age 18-40 years
• Healthy
• Stable dietary habits
• No use of medication
• Lean subjects: BMI 18-25 kg/m2
• Obese subjects: BMI 30-35 kg/m2

Exclusion criteria

• Any medical condition requiring treatment and/or medication use

• Alcohol consumption of more than 20 g per day (± 2 units)

• Smoking

• Unstable body weight (weight gain or loss > 3 kg in the past three months)

• Participation in another biomedical study within 1 month prior to the screening visit

• Contraindications for MRI scan:

  • Central nervous system aneurysm clips
  • Implanted neural stimulator
  • Implanted cardiac pacemaker of defibrillator
  • Cochlear implant
  • Iron- containing corpora aliena in the eye or brain
  • Hearing aids and artificial (heart) valves which is contraindicated for MRS
  • Claustrophobia

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: Single Blind

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9 participants in 4 patient groups

High fat breakfast lean subjects

Other group

Description:

Subjects will consume a high fat breakfast, containing of sausage rolls. Energy content of the breakfast will be set to 50 % of the recommended daily calorie intake (BMR times 1.3).

Treatment:

Dietary Supplement: High fat breakfast

High fat breakfast,obese subjects

Other group

Description:

Subjects will consume a high fat breakfast, containing of sausage rolls. Energy content of the breakfast will be set to 50 % of the recommended daily calorie intake (BMR times 1.3).

Treatment:

Dietary Supplement: High fat breakfast

HF breakfast with protein,lean subjects

Other group

Description:

For the HFP breakfast, the subjects will consume 'Protifar' (Nutricia, Cuijk, The Netherlands) in addition to the sausage rolls. The amount of added proteins will be calculated to assure that the total energy content from proteins in the breakfast equals 20 %,

Treatment:

Dietary Supplement: HF breakfast with protein

HF breakfast with protein,obese subjects

Other group

Description:

For the HFP breakfast, the subjects will consume 'Protifar' (Nutricia, Cuijk, The Netherlands) in addition to the sausage rolls. The amount of added proteins will be calculated to assure that the total energy content from proteins in the breakfast equals 20 %,

Treatment:

Dietary Supplement: HF breakfast with protein