Preliminary Study of Sonic Hedgehog Signaling Pathway in the Pathogenesis of Rheumatoid Arthritis

Sun Yat-sen University

Status

Unknown

Conditions

Synovitis

Neovascularization, Pathologic

Arthritis, Rheumatoid

Study type

Observational

Funder types

Other

Identifiers

NCT01731262

中大附三医伦[2012]2-75号

Details and patient eligibility

About

Rheumatoid arthritis(RA) with a high incidence and high morbidity, the pathogenesis has not been fully elucidated. Fibroblast-like synovial cells excessive proliferation and synovial angiogenesis is the most important cause of RA synovitis and joint destruction. Our study was to find the role of Sonic Hedgehog(SHH) pathway in regulating proliferation of fibroblast-like synovial cells and modulating excess angiogenesis of synovial tissue.

Full description

4ml blood from active RA patients(n=35) and healthy volunteers (n=35) will be collected

peripheral blood mononuclear cells will be detached
messenger ribonucleic acid expression of Sonic Hedgehog pathway associated factors in both groups will be detected

Synovial tissues from 4 RA patients and 4 patients with traumatic or meniscal injury who need to carry out knee joint replacement operation will be collected

inflammatory feature of synovial tissue will be observed
the expression of Shh, Ptch1, Gli1 and Smo in synovial tissue will be detected by immunohistochemistry assay
fibroblast-like synoviocytes will be cultured from synovial tissue of RA patients

Enrollment

78 estimated patients

Sex

All

Ages

18 to 65 years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria