Project A3sc - An Atlas of Airways at a Single Cell Level (AAAsc)

C

Centre Hospitalier Universitaire de Nice

Status

Completed

Conditions

Respiratory System

Treatments

Procedure: Bronchoscopy

Study type

Interventional

Funder types

Other

Identifiers

NCT03437122
17-PP-14

Details and patient eligibility

About

The increasing incidence of chronic respiratory diseases is a public health problem affecting hundreds of thousands of people around the world, including children. Directly exposed to atmospheric aerocontaminants (pollution, allergens), the respiratory tracts represent a complex ecosystem that involves different cells that develop complex interactions with the surrounding connective tissue but also with their rich immune environment and with the microbiota. Although a pathophysiological continuum is postulated between the nasal and bronchial airways in certain diseases, such as allergic diseases, we have identified broad gradients in gene expression between nasal and bronchial samples. This is why cellular variability throughout the respiratory tree needs to be studied in detail. The sequencing of RNAs specifically present in a particular cell, and its comparison with neighboring cells, allows us to document precise cellular contributions and intercellular relationships. Our project will establish protocols to stabilize airway swabs by brushing and/or biopsy, under conditions that will then allow the analysis of gene expression profiles at the single cell level (single cell RNA sequencing). The development of the "single cell" stabilisation and analysis protocol will first be carried out on primary respiratory epithelium cultures and then extended to respiratory specimens taken from healthy volunteers. Through sampling at several levels of the respiratory tree, variations in expression along the tracheobronchial axis will be fully documented. Finally, the interaction between the epithelial compartment and the immunological compartment will be studied by analyzing gene expression on a single cell in different physiopathological contexts.

Enrollment

15 patients

Sex

All

Ages

18 to 40 years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

  • age ≥ 18 years and <40 years
  • non-smoker
  • absence of known acute or chronic disease
  • absence of symptoms suggestive of progressive disease
  • normal clinical examination

Exclusion criteria

  • recent rhino-bronchial infection (<6 weeks)
  • mental disability
  • pregnant women (a urine test will be performed for all women of childbearing age)

Trial design

Primary purpose

Basic Science

Allocation

N/A

Interventional model

Single Group Assignment

Masking

None (Open label)

15 participants in 1 patient group

All
Experimental group
Treatment:
Procedure: Bronchoscopy

Trial contacts and locations

1

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Data sourced from clinicaltrials.gov

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