Protein and RNA Expression Patterns in Predicting Response to Treatment in Patients With Lung Cancer

Vanderbilt University Medical Center

Status: Completed

Conditions: Lung Cancer

Treatments: Genetic: DNA/RNA sequencing and expression levels; Procedure: Blood sample; Procedure: Lung tumor biopsy; Genetic: Protein expression analysis

Study type: Observational

Funder types: Other; NIH

Identifiers

NCT00897650

VICC THO 0547

P50CA090949 (U.S. NIH Grant/Contract)

P30CA068485 (U.S. NIH Grant/Contract)

VU-VICC-THO-0547 (Other Identifier)

Details and patient eligibility

About

RATIONALE: Studying samples of tumor tissue and blood in the laboratory from patients with cancer may help doctors learn more about changes that occur in genetic material (DNA and RNA) and may also identify protein expression patterns related to cancer. It may also help doctors predict how patients will respond to treatment.

PURPOSE: This research study evaluates changes in DNA, RNA, and proteins with the goal of predicting response to treatment in patients with lung cancer.

Full description

OBJECTIVES:

• To determine protein and/or RNA expression patterns capable of predicting tumor response to therapy in tumor tissue samples from patients with lung cancer or suspected of having lung cancer.
• To characterize the genes and proteins found to be predictive of response in order to help elucidate the molecular biology underlying cancer chemosensitivity.
• To evaluate DNA mutations found within the lung cancer sample which may be predictive of response or resistance to certain therapeutic agents.

OUTLINE: Patients undergo collection of tumor tissue by percutaneous fine needle aspiration, core biopsy, thoracentesis, or during any medically indicated procedure involving removal of lung cancer tissue. Tissue samples are analyzed by a variety of techniques, including DNA sequencing, RNA sequencing and expression levels, protein assessment [by immunohistochemistry, western blot, Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-MS]). The goal of these studies is to identify of gene mutations, gene expression levels, and proteins predictive of treatment response. Blood samples are also collected to obtain normal DNA for analysis.

After completion of study, patients will be followed until their death.

Enrollment: 204 patients

Sex: All

Ages: Under 120 years old

Volunteers: No Healthy Volunteers

Inclusion and exclusion criteria

Inclusion criteria

• Diagnosis of suspected lung cancer or lung cancer

Exclusion criteria

• Inability to undergo therapy

Trial design

204 participants in 1 patient group

Lung cancer

Description:

Patients with a diagnosis of invasive lung cancer.

Treatment:

Genetic: Protein expression analysis

Genetic: DNA/RNA sequencing and expression levels

Procedure: Blood sample

Procedure: Lung tumor biopsy