Protein Supplementation and Endurance Exercise Adaptations

Wageningen University

Status

Completed

Conditions

Exercise

Treatments

Dietary Supplement: Protein

Study type

Interventional

Funder types

Other

Identifiers

NCT03462381

NL5988508116

Details and patient eligibility

About

Participants will perform three endurance training sessions weekly for 12 weeks. All participants will be randomly assigned to the protein supplementation group or the placebo (iso-caloric carbohydrate) group. During the 12 weeks endurance training program participants allocated to the protein group (30 g protein per beverage) will receive a protein drink after exercise and prior to sleep at night on training days. On non-training days participants will only receive a protein drink before sleep. Participants allocated to the carbohydrate group (30 g carbohydrates) will receive a carbohydrate drink instead of a protein drink.

Full description

In the proposed study, the effects of protein supplementation after training and before sleep on VO2max and performance will be assessed during 12 weeks endurance exercise training in untrained healthy young men. The ready to drink carbohydrate beverage consists of 30g carbohydrate or 30g protein. Previous studies show that the supplementation of 30g protein is in the optimal range to stimulate the muscle protein synthetic response following resistance type exercise. The protein supplementation group in the present study will be compared with an iso-caloric carbohydrate group. In addition, to the pre- and post-intervention biopsies 1 additional muscle biopsies will be taken (in week 6) during the 12 week intervention period. This will create a unique opportunity to establish the timeline of the skeletal muscle adaptive response at functional and mechanistic level following prolonged endurance exercise training, including potential differences between both groups.

Enrollment

44 patients

Sex

Male

Ages

18 to 30 years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

Male
Age between 18 and 30 years of age
BMI between 18.5 - 25 kg/m2
Recreationally active, performing sports on a non-competitive basis
Maximal wattage of > 5 watt per kg bodyweight (screening measurement)
Willing to give muscle biopsies
Willing to give blood samples
Having a general-practitioner
Consumption of alcohol beverages is less than 21 per week
Able to perform three exercise sessions weekly for 10 weeks

Exclusion criteria

Medical condition that can interfere with the study outcome (i.e. cardiovascular disease, pulmonary disease, liver or renal disease, diabetes mellitus type 1 or 2)
Having a lactose and/or gluten intolerance
Use of systemic medication (with the exception of antihistaminic medication and the use of occasional painkillers)
(Chronic) injuries of the locomotor system (e.g. musculoskeletal/orthopedic disorders) that can interfere with the intervention
Participants who are enrolled in an interventional biomedical research project or have received an investigational new drug or product with the last 30 days prior to screening.
Smokers and use of illicit drugs
Blood donor during the study and in the three months before start of the study
Employed, or intern, or working on a thesis at the department of Human Nutrition at Wageningen University & Research
Participating in another scientific study (except EetMeetWeet)