Protein Supplementation and Skeletal Muscle Healing Process

University of Thessaly

Status: Completed

Conditions: Exercise-induced Aseptic Inflammation; Skeletal Muscle Performance; Proteasome Activation; Intracellular Signaling in Skeletal Muscle; Skeletal Muscle Damage

Treatments: Dietary Supplement: Placebo; Dietary Supplement: Milk protein isolate

Study type: Interventional

Funder types: Other

Identifiers

NCT02816411

PROTEIN-MUSCLEINJURY-2014

Details and patient eligibility

About

In this study the investigators utilized protein supplementation over an 8-day period following eccentric exercise-induced muscle damage in order to test the initial hypotheses : i) protein supplementation after exercise-induced muscle injury affects exercise-induced aseptic inflammation and muscle performance.

Full description

The objective was to examine weather protein supplementation is able to affect the inflammatory response as well as recovery of muscle performance following an intense eccentric exercise protocol. In a double-blind, counterbalanced design, 14 men received either Placebo (PLA) or milk protein isolate (PRO) for 8 consecutive days following a single bout of exercise (300 eccentric contractions at 30 deg/sec). In both conditions, performance was assessed at baseline, immediately post-exercise, 2h post-exercise and daily for 8 consecutive days. Blood samples were collected at baseline, 2h post-exercise and daily for the remaining 8 days. Muscle biopsies from vastus lateralis were collected at baseline as well as at day 2 and day 8 of the post-exercise period.

Enrollment: 14 patients

Sex: Male

Ages: 18 to 30 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• a) recreationally trained as indicated by the maximal oxygen consumption levels (VO2max > 45 ml/kg/min), b) engaged in systematic exercise at least three times per week for > 12 months, c) non-smokers, d) abstained from any vigorous physical activity during the study, e) abstained from consumption of caffeine, alcohol, performance-enhancing or antioxidant supplements, and medications during the study.

Exclusion criteria

• a) a recent febrile illness, b) history of muscle lesion, c) lower limb trauma

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: Double Blind

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14 participants in 2 patient groups

Protein

Experimental group

Description:

Milk protein isolate supplementation: orally, 20g of protein immediately post-exercise and then 20g every 3h on 3 occasions (+3, +6, +9), on the exercise day. The remaining 8 days, 20g daily with breakfast.

Treatment:

Dietary Supplement: Milk protein isolate

Placebo

Active Comparator group

Description:

Placebo administration: orally 500 ml, immediately post-exercise as well as at +3, +6 and +9 hours, on the exercise day. The remaining 8 days, 500 ml daily with breakfast.

Treatment:

Dietary Supplement: Placebo