Proteomic Analysis of Crohn's Disease Macrophages in Response or Not to AIEC (ROMAN)

University Hospital, Clermont-Ferrand

Status

Completed

Conditions

Ulcerative Colitis

Crohn's Disease

Treatments

Other: Proteomic analysis

Study type

Observational

Funder types

Other

Identifiers

NCT03404557

2017-A00667-46 (Other Identifier)

CHU-370

Details and patient eligibility

About

The M2iSH laboratory showed with two previous clinical trials that Crohn's Disease (CD) macrophages present i) a defect to control Adherent-Invasive Escherichia coli (AIEC) infection related to polymorphisms associated with CD; ii) a CD - specific cytokine secretion profile after an AIEC infection and intestinal inflammation dependent; iii) a modification of the response of CD macrophages at a basal state and after the AIEC infection. These results consolidate the hypothesis of a defect specific to CD macrophages.

That's why, the primary purpose of this study is to realize a proteomic analysis of macrophages of CD patients infected or not with AIEC and to compare them to Ulcerative Colitis (UC) patients and healthy volunteers.

Full description

The macrophages characterization will be realized at a basal state and in response or not to AIEC.

Investigator aimed to 1) better understand the differences between macrophages of CD patients, UC patients and healthy volunteers but also the impact of AIEC infection on these macrophages to provide key informations to detect and characterize the defect of these macrophages; 2) highlight one or several protein in CD macrophages that could represent, in a long term, a potential therapeutic target of CD.

Enrollment

88 patients

Sex

All

Ages

18+ years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

- Crohn's Disease or ulcerative colitis or healthy volunteers
Age > 18 years
Patients benefiting from the health insurance plan
Patients who can read and attest to understanding the information note and informed consent

Exclusion criteria