Proteomic Analysis of Mononuclear Cells After a High-Fat, High-Carbohydrate Meal With or Without Orange Juice

University of Sao Paulo

Status: Completed

Conditions: Inflammation

Treatments: Other: Water with glucose; Other: Orange juice; Other: Water

Study type: Interventional

Funder types: Other

Identifiers

NCT02587507

TMTMNC

Details and patient eligibility

About

A high-fat, high-carbohydrate meal (HFHC) induces an increase in inflammation in peripheral-blood mononuclear cells in healthy subjects. A few studies have shown that orange juice, when consumed together with the high-fat meal, is able to revert the increase in inflammatory markers. The present study will assess the effect of a single HFHC meal taken with water, orange juice or an isocaloric glucose drink on protein expression in peripheral blood mononuclear cells of healthy individuals. Twelve healthy, normal weight individuals consumed a HFHC meal with water, water with glucose or orange juice in a cross-over design with at least one week interval between the meals. Blood samples were collected before, 1, 3 and 5h after the meals.

Full description

BACKGROUND:

A high-fat, high-carbohydrate meal (HFHC) induces an increase in inflammation in peripheral-blood mononuclear cells in healthy subjects. A few studies have shown that orange juice, when consumed together with the high-fat meal, is able to revert the increase in inflammatory markers.

OBJECTIVES:

To study the effect of a single HFHC meal taken with water, orange juice (500ml) or an isocaloric glucose drink on protein expression in peripheral blood mononuclear cells of healthy individuals.

DESIGN AND METHODOLOGY:

Twelve healthy, normal weight individuals consumed a HFHC meal with water, water with glucose or orange juice in a cross-over design with at least one week interval between the meals. Blood samples were collected before, 1, 3 and 5h after the meals. Blood glucose, insulin and lipid profile were measured. Also, proteome analysis using TMT labeling was performed on peripheral-blood mononuclear cells collected before and at times 3 and 5h after the meal.

Enrollment: 12 patients

Sex: All

Ages: 20 to 35 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• healthy
• normal weight
• non-vegetarian

Exclusion criteria

• use of medication
• alcohol consumption,
• intense physical exercise
• pregnant or lactating
• overweight
• obese
• drug abuse
• any disease such as diabetes, inflammatory conditions.

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: None (Open label)

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12 participants in 3 patient groups, including a placebo group

Water

Placebo Comparator group

Description:

The subjects will ingest the HFHC meal with 500mL of water.

Treatment:

Other: Water

Orange juice

Experimental group

Description:

The subjects will ingest the HFHC meal with 500mL of orange juice.

Treatment:

Other: Orange juice

Water with glucose

Active Comparator group

Description:

The subjects will ingest the HFHC meal with 500mL of water with glucose (isocaloric control of the orange juice).

Treatment:

Other: Water with glucose