Proteomic Analysis of Patients Undergoing Bariatric Surgery

Universidade Federal de Pernambuco

Status: Not yet enrolling

Conditions: Breast Cancer; Esophageal Cancer; Colon Cancer; Obesity; Ovarian Cancer; Liver Cancer; Endometrial Cancer; Gallbladder Cancer; Potmenopausal Breast Cancer; Multiple Myeloma; Meningioma; Gastric Cancer; Kidney Cancer; Thyroid Cancer; Ovarian; Prostate Cancer; Pancreatic Cancer

Treatments: Procedure: bariatric surgery

Study type: Interventional

Funder types: Other

Identifiers

NCT06787807

78992524100005208

Details and patient eligibility

About

The expression of proteins in the blood of obese individuals is different from the expression of proteins in healthy individuals, and is also different in individuals after bariatric surgery. Therefore, this research aims to better understand protein expression in patients indicated for surgical treatment of obesity, evaluating the pre-operative and post-operative status. The objective of this research is to better understand protein expression in patients indicated for surgical treatment of obesity, evaluating the pre-operative and post-surgery status.

Full description

The objective of this study is to analyze the Proteomics of obese patients in the preoperative context of bariatric surgery to evaluate which are the up-regulated proteins - potentially related to oncogenic metabolic pathways - and also the down-regulated proteins in the context of obesity - potentially protective in the context of cancer. As well as, evaluate the variation of these proteins, as well as the metabolic pathways with which they are associated, after weight loss promoted by bariatric surgery, in the period of 01 year after this intervention.

The proteomic study will be carried out based on the formation of pools organized based on the patients' pre- and postoperative similarities. Proteomic analyzes will be carried out on a high-performance liquid chromatography system (nanoUPLC) model Acquity Mclass, Waters. Coupled to a Quadrupole Mass Spectrometer-Time of Flight (QToF) Model Sunapt Xs, Waters. Finally, the raw data (raw mass spectra) will be analyzed by the PROGENESIS QI software (Waters) to identify the proteins.

Enrollment: 103 estimated patients

Sex: All

Ages: 18+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• patients over 18 years of age with a BMI above 35 kg/m² associated with comorbidity or BMI above 40 kg/m² without comorbidities,

Exclusion criteria

• Patients with a previous history of cancer;
• Underage patients
• Patients with other diseases predisposing to the development of cancer: cirrhosis, ulcerative colitis, Barrett's esophagus, etc.
• Patients who have previously undergone bariatric surgery;

Trial design

Primary purpose: Prevention

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)

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103 participants in 7 patient groups

women with insulin resistance

Experimental group

Description:

An analysis was made of patients with severe steatosis, among patients undergoing bariatric surgery

Treatment:

Procedure: bariatric surgery

patients with severe steatosis

Experimental group

Description:

An analysis was made of patients with severe steatosis, among patients undergoing bariatric surgery

Treatment:

Procedure: bariatric surgery

General patients with insulin resistance

Experimental group

Description:

An analysis was made of patients, among men and women, with insulin resistance, among patients undergoing bariatric surgery

Treatment:

Procedure: bariatric surgery

Patients with above average CRP

Experimental group

Description:

An analysis was carried out of patients with CRP above the average, among patients undergoing bariatric surgery

Treatment:

Procedure: bariatric surgery

Patients with grade 1 obesity

Experimental group

Description:

An analysis was made of patients with grade 1 obesity, among patients undergoing bariatric surgery

Treatment:

Procedure: bariatric surgery

Patients with grade 2 obesity

Experimental group

Description:

An analysis was made of patients with grade 2 obesity, among patients undergoing bariatric surgery

Treatment:

Procedure: bariatric surgery

Patients with grade 3 obesity

Experimental group

Description:

An analysis was made of patients with grade 3 obesity, among patients undergoing bariatric surgery

Treatment:

Procedure: bariatric surgery