Proteomic and Metabolomic Lacrimal Fingerprint in Diverse Pathologies of the Ocular Surface (EML-MSO)

Centre hospitalier de l'Université de Montréal (CHUM)

Status: Enrolling

Conditions: Infectious Keratoconjunctivitis; Allergic Conjunctivitis; Mucous Membrane Pemphigoid; Dry Eye Syndrome

Treatments: Diagnostic Test: Tear sample collection via Schirmer strip and subsequent analysis by mass spectrometry

Study type: Observational

Funder types: Other

Identifiers

NCT04198740

CE19.212

Details and patient eligibility

About

This study aims to obtain the lacrimal fingerprint for frequent pathologies of the ocular surface and establish a normative base for each of them.

Full description

This is an exploratory study. The investigators aim to:

• Test and perfect a tear sample analysis technique by mass spectrometry with samples collected by Schirmer strips;
• Obtain the lacrimal fingerprint for various pathologies of the ocular surface, notably different forms of dry eye syndrome, infectious keratitis/conjunctivitis, mucous membrane pemphigoid and allergic conjunctivitis, and establish a normative base for each of them.

During regular clinics at the cornea service of the ophthalmology department of the CHUM (Centre Hospitalier de l'Université de Montréal), the investigators aim to recruit a few hundred patients with various pathologies of the ocular surface, notably different forms of dry eye syndrome, infectious keratitis/conjunctivitis, mucous membrane pemphigoid and allergic conjunctivitis, and collect a sample of their tears via Schirmer strip. A case report form will be completed for each patient, noting known ocular diagnoses and active topical ophthalmic medication. Schirmer strips will be sent in sterile tubes to UQAM (Université du Québec à Montréal)'s department of chemistry for analysis by mass spectrometry.

Enrollment: 300 estimated patients

Sex: All

Ages: 18+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Patients with healthy corneas or suffering from one of these pathologies:

Dry eye syndrome; Infectious keratitis and/or conjunctivitis; Mucous membrane pemphigoid; Allergic conjunctivitis.

Exclusion criteria

• Patients younger than 18 years old;
• Patients incapable of giving informed consent.

Trial design

300 participants in 5 patient groups

Control group

Description:

Healthy, normal ocular surface

Treatment:

Diagnostic Test: Tear sample collection via Schirmer strip and subsequent analysis by mass spectrometry

Dry eye syndrome

Description:

Patients suffering from either: * Lacrimal insufficiency * Anterior blepharitis * Posterior blepharitis * Sjögren syndrome

Treatment:

Diagnostic Test: Tear sample collection via Schirmer strip and subsequent analysis by mass spectrometry

Allergic conjunctivitis

Description:

Patients suffering from allergic conjunctivitis

Treatment:

Diagnostic Test: Tear sample collection via Schirmer strip and subsequent analysis by mass spectrometry

Mucous membrane pemphigoid

Description:

Patients suffering from mucous membrane pemphigoid

Treatment:

Diagnostic Test: Tear sample collection via Schirmer strip and subsequent analysis by mass spectrometry

Infectious keratoconjunctivitis

Description:

Patients suffering from keratitis and/or conjunctivitis of various etiology: * Viral * Bacterial * Fungal * Acanthamoeba

Treatment:

Diagnostic Test: Tear sample collection via Schirmer strip and subsequent analysis by mass spectrometry