Randomized Trial With Dendritic Cells in Patients With Metastatic Colorectal Cancer

Fundacion Clinic per a la Recerca Biomédica

Status and phase

Completed

Phase 2

Conditions

Colorectal Neoplasms

Treatments

Drug: Dendritic Cell Vaccine

Other: Supportive treatment

Study type

Interventional

Funder types

Other

Identifiers

NCT01413295

TRA-082 (Other Grant/Funding Number)

MCBRVP

2009-017247-33 (EudraCT Number)

Details and patient eligibility

About

The different alternatives used since 1996 to treat metastatic colorectal cancer (MCRC) have increased the mean survival of these patients. This outstanding advance is due to the extended indications for resection of hepatic metastases and to the use of new chemotherapeutic drugs (fluoropyrimidine, irinotecan and oxaliplatin) and monoclonal antibodies (bevacizumab, cetuximab and panitumumab). However, none of these treatments is curative and the majority of patients are overwhelmed by the illness. The first line of treatment for MCRC is FOLFOX and the second, irinotecan plus cetuximab for patients with wild type KRAS gene (60%) with a 30% responses, and bevacizumab plus irinotecan with a 5-10% of responses, in patients with mutated KRAS (40%). A treatment with autologous dendritic cells (DCs) pulsed with autologous tumour antigens is proposed as a third line of therapy. A randomized phase II trial would be performed, by selecting two groups of patients, one of them would be treated with the best supportive treatment and the other with DCs plus the best supportive treatment. The aim of the study would be to analyze the outcome after 4 months of treatment. In patients treated with DCs, IFN-γ spot forming cells and proliferative responses would be determined pre and post treatment in lymphocytes stimulated with autologous DCs pulsed with autologous tumour antigens. Pre and post treatment serum levels of IFN-γ, TNF-α, TGF-β e IL-12, would also be measured.

Enrollment

52 patients

Sex

All

Ages

18+ years old

Volunteers

No Healthy Volunteers

Inclusion criteria

Age over 18 years.
Capacity of understanding and signing the informed consent and to undergo the study procedures.
Previously treated with 2 lines of chemotherapy.
ECOG <= 2.
Adequate renal, hepatic and bone marrow function
Confirmed diagnosis of colorectal cancer with hepatic metastasis, suitable for biopsy.
Availability of tumor tissue, for maturing dendritic cells
RECIST.1 criteria

Exclusion criteria

Clinically relevant diseases or infections (HBV, HCV, HIV).
Pregnant or breast feeding women.
Immunosuppressant treatment.
Concurrent cancer, with the exceptions allowed by the principal investigator (PI).