Rate-limiting Amino Acids in Endurance-trained Athlete (EA)

University of Toronto

Status: Completed

Conditions: Healthy; Increased Metabolic Requirement

Treatments: Dietary Supplement: Sufficient protein; Dietary Supplement: Non essential amino acids; Dietary Supplement: Branched chain amino acids; Dietary Supplement: Base protein; Dietary Supplement: Essential amino acids

Study type: Interventional

Funder types: Other

Identifiers

NCT02628249

IAAO-EA

Details and patient eligibility

About

Protein requirements in individuals who participate in endurance-based exercise training have been suggested to be greater than the current recommended dietary allowance (RDA).

The biological value of protein depends on the amino acid composition. As liebig's law of the minimum, the protein synthesis is limited due to the lack of single rate limiting amino acid. In this case, the first rate limiting amino acid determines protein intakes to maximize the protein synthesis. Adding a small amount of rate-limiting amino acid to the diet improves the biological value of its protein diet.

The indicator amino acid oxidation (IAAO) method has clarified the individual amino acid requirement in children, normal healthy adult and clinical populations. however, the IAAO method has never been utilized for determining the first rate limiting amino acid.

Therefore, the purpose of this study is to address of IAAO method to be applied for determining the rate-limiting amino acid in endurance athlete.

Enrollment: 5 patients

Sex: Male

Ages: 18 to 35 years old

Volunteers: Accepts Healthy Volunteers

Inclusion and exclusion criteria

Gender: Male

Age limit: Minimum age 18 years - Maximum age 35 years

Accepts Healthy Volunteers : yes

Inclusion Criteria:

• Endurance-trained participants who regularly more than 40 km/week
• Ability to perform the exercise stimulus (20 km run) on metabolic trial.

Exclusion Criteria:

• Inability to meet health and physical activity guidelines according to the physical activity readiness questionnaire (PAR-Q+)
• Inability to adhere to any of the protocol guidelines (i.e. alcohol, caffeine consumption)
• Regular tobacco use
• Illicit drug use

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: Single Blind

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5 participants in 5 patient groups

Base protein

Experimental group

Description:

0.8 g/kg/d of protein provided as crystalline amino acid made after egg protein.

Treatment:

Dietary Supplement: Base protein

Sufficient protein

Experimental group

Description:

1.75 g/kg/d protein provided as crystalline amino acid made after egg protein.

Treatment:

Dietary Supplement: Sufficient protein

Base + BCAA

Experimental group

Description:

Base protein intake + Branched chain amino acids

Treatment:

Dietary Supplement: Base protein

Dietary Supplement: Branched chain amino acids

Base + EAA

Experimental group

Description:

Base protein intake + essential amino acids.

Treatment:

Dietary Supplement: Base protein

Dietary Supplement: Essential amino acids

Base + NEAA

Experimental group

Description:

Base protein intake + non essential amino acids

Treatment:

Dietary Supplement: Base protein

Dietary Supplement: Non essential amino acids