Regeneration of Bronchial Epithelium During Chronic Obstructive Pulmonary Disease (EPITHREGENCOPD)

CHU de Reims

Status: Completed

Conditions: COPD

Treatments: Other: bronchial epithelial cell culture

Study type: Observational

Funder types: Other

Identifiers

NCT02873988

PA11017

Details and patient eligibility

About

Airway epithelium integrity is essential to maintain its role of mechanical and functional barrier. Recurrent epithelial injuries require a complex mechanism of repair to restore its integrity. In chronic obstructive pulmonary disease (COPD), an abnormal airway epithelial repair may participate in airway remodelling. The objective was to determine if airway epithelial wound repair of airway epithelium is abnormal in COPD.

Full description

Patients scheduled for lung resection were prospectively recruited. Demographic, clinical data and pulmonary function tests results were recorded. Emphysema was visually scored and histological remodelling features were noted. Primary bronchial epithelial cells (BEC) were extracted and cultured for wound closure assay. The investigators determined the mean speed of wound closure (MSWC) and cell proliferation index, matrix metalloprotease (MMP)-2, MMP-9 and cytokines levels in supernatants of BEC 18 hours after cell wounding. In a subset of patients, bronchiolar epithelial cells were also cultured for wound closure assay for MSWC analyze.

Enrollment: 25 patients

Sex: All

Ages: 40+ years old

Volunteers: No Healthy Volunteers

Inclusion criteria

6 Patients scheduled for lung resection for cancer

Exclusion criteria

• Patients with asthma, cystic fibrosis, bronchiectasis or pulmonary fibrosis were excluded

Trial design

25 participants in 2 patient groups

patients with COPD

Description:

patients with COPD

Treatment:

Other: bronchial epithelial cell culture

patients without COPD

Description:

patients without COPD

Treatment:

Other: bronchial epithelial cell culture