Regeneration of Posterior Cruciate Ligament Injury Using Hypoxic Conditioned Allogenic Adipose Mesenchymal Stem Cell and Condition Medium

Gadjah Mada University

Status and phase: Unknown; Early Phase 1

Conditions: Ligament Rupture; Ligament Derived Conditined Medium; Adypose Mesenchimal Stem Cell

Treatments: Biological: adypose mesenchymal conditioned medium and ligament derived conditioned medium

Study type: Interventional

Funder types: Other

Identifiers

NCT04889963

STEMCELL-LIGAMENTREGENERATION

Details and patient eligibility

About

Transplantation of Allogenic Adipose Mesenchimal Stem cell in hypoxic cultur condition with Ligament- derived conitioned medium can enhance regeneration of posterior cruciate ligament rupture

Full description

this study is in vivo study to prove effect of transplantation of Allogenic Adipose Mesenchimal Stem cell in hypoxic cultur condition with Ligament- derived conitioned medium in regeneration of posterior cruciate ligament rupture

Enrollment: 16 estimated patients

Sex: Male

Ages: 2 to 12 months old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• white male new zealand rabbit
• weight 2500 - 3000 gram

Exclusion criteria

• have no disease

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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16 participants in 4 patient groups, including a placebo group

control group

Placebo Comparator group

Description:

rabbit with posterior cruciate ligament rupture and treated with fibrin glue (sacffold)

Treatment:

Biological: adypose mesenchymal conditioned medium and ligament derived conditioned medium

conditioned medium group

Active Comparator group

Description:

rabbit with posterior cruciate ligament rupture and treated with fibrin glue mix with ligament derived conditioned medium

Treatment:

Biological: adypose mesenchymal conditioned medium and ligament derived conditioned medium

stem cells group

Active Comparator group

Description:

rabbit with posterior cruciate ligament rupture and treated with fibrin glue mix with adypose mesenchymal stem cells in hypoxic culture condition

Treatment:

Biological: adypose mesenchymal conditioned medium and ligament derived conditioned medium

composite group

Experimental group

Description:

rabbit with posterior cruciate ligament rupture and treated with fibrin glue mix with adypose mesenchymal stem cells in hypoxic culture condition and ligament derived conditioned medium

Treatment:

Biological: adypose mesenchymal conditioned medium and ligament derived conditioned medium