Regulation of Tissue Lipolysis by Insulin in Type 2 Diabetes

Maastricht University Medical Centre (MUMC)

Status: Completed

Conditions: Type 2 Diabetes

Treatments: Other: Hyperinsulinemic euglycemic clamp

Study type: Observational

Funder types: Other

Identifiers

NCT01680133

91611074 (Other Grant/Funding Number)

07-3-037

Details and patient eligibility

About

Inadequate suppression of intramuscular and adipose tissue lipolysis, and consequent excessive delivery of fatty acids to ectopic tissues (e.g. muscle, pancreas and liver) could play an important role in the development and exacerbating of insulin resistance. Therefore, the investigators propose to study the regulation of adipose tissue and skeletal muscle lipolysis, as well as further characterize the intracellular lipolytic pathways within these tissues, in obese normoglycaemic versus long-term diagnosed type 2 diabetic subjects.

Enrollment: 20 patients

Sex: Male

Ages: 45 to 65 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Caucasian
• Normal blood pressure (SBP 100-140 mmHg, DBP 60-90 mmHg)
• weight stable in last 3 months

Exclusion criteria

• Smokers
• people with intensive fitness training (e.g. athletes > 3 times/week)
• History of cardiovascular diseases
• Bleeding disorders
• Use of medication interfering with the study endpoints/hypotheses (e.g. beta-blockers)
• Not to be able to understand the study information
• Subjects on a special diet or vegetarian
• Blood donation 2 months prior to the study and during the study
• Participating in an other study

Trial design

20 participants in 2 patient groups

NGT

Description:

Normal glycaemic healthy control men, age between 45-65, BMI 25-35 kg/m2

Treatment:

Other: Hyperinsulinemic euglycemic clamp

T2D

Description:

Type 2 diabetic men, age 45-65 yrs, BMI 25-35, diagnosed \>5yrs and Hba1c 7-9%

Treatment:

Other: Hyperinsulinemic euglycemic clamp