Relation Between MicroRNA 203 and 210 and Sparing the Laparoscopic Examination in Cases of Unexplained Infertility

Benha University

Status: Completed

Conditions: Infertility Unexplained; Endometriosis

Treatments: Diagnostic Test: Estimation of Plasma Expression level of MICRO RNA 203 & 210; Procedure: Laparoscopic Exploration

Study type: Interventional

Funder types: Other

Identifiers

NCT05680350

RC 2.6.2022

Details and patient eligibility

About

The study targets to evaluate the role of estimation of plasma expression levels of gene of microRNAs 203 and 210 in helping to diagnose and grade endometriosis. The study included women with unexplained infertility and clinical picture suggestive of presence of endometriosis were divided randomly into two groups: Control and study groups. All patients underwent exploratory laparotomy for diagnosis and grading of endometriosis if present. Patients of study group gave blood samples for estimation of plasma expression levels of microRNA 203 and 210 using qRT-PCR procedure.

Enrollment: 100 patients

Sex: Female

Ages: 30 to 45 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• 30-45 years old
• Unexplained infertility

Exclusion criteria

• Presence of other causes of infertility,
• age out of the predetermined range,
• the presence of immune disorders,
• maintenance on immunosuppressive drugs

Trial design

Primary purpose: Diagnostic

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Single Blind

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100 participants in 2 patient groups

Study group

Active Comparator group

Description:

Study group Patients with unexplained infertility had estimated their Plasma Expression level for Gene"Micro RNA "203 \& 210" by qRT-PCR followed by laparoscopic exploration

Treatment:

Diagnostic Test: Estimation of Plasma Expression level of MICRO RNA 203 & 210

Procedure: Laparoscopic Exploration

Control Group

Active Comparator group

Description:

Control group Patients with unexplained infertility underwent laparoscopy only

Treatment:

Procedure: Laparoscopic Exploration