Relationship Between Polymorphism of Heat Shock Protein 70 Gene and Hepatocellular Carcinoma (HSP70)

Kaohsiung Medical University

Status: Completed

Conditions: Hepatocellular Carcinoma

Treatments: Genetic: Polymorphisms of HSP70 and TNF promoter

Study type: Interventional

Funder types: Other

Identifiers

NCT00769535

KMUH-IRB-930047

Details and patient eligibility

About

Polymorphisms of HSP70 and tumor necrosis factor-alpha promoter in patients with hepatocellular carcinoma, chronic liver disease and healthy controls will be measured by PCR-RFLP or direct sequencing. The clinical relevance of patients will be compared in those with polymorphism and those without.

Full description

Heat shock protein 70 (HSP 70) may be involved in various aspects of immune system including infection, autoimmunity, and tumor immunity. The relationship between HSP70 expression, disease activity and cancer prognosis varied according to type of cancer. The overexpression of HSP70 by tumor cells contributes to tumor behavior. HSP70 is present in normal and abnormal hepatocytes, and was reported to be over-expressed in both hepatoma cell lines and human hepatocellular carcinoma (HCC) tissue. Recent study indicated that HSP70 is a molecular marker during early hepatocarcinogenesis. It is also involved in anti-tumor immunity in HCC.We also investigate the polymorphism of tumor necrosis factor-alpha promoter, and correlated to clinical relevance.

Enrollment: 600 patients

Sex: All

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• History proven HCC patients

Exclusion criteria

• No-definite diagnosed patients

Trial design

Primary purpose: Basic Science

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)

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600 participants in 1 patient group

HSP70 and TNF polymorphisms

Experimental group

Treatment:

Genetic: Polymorphisms of HSP70 and TNF promoter