Relationship Between the VEGF Gene Polymorphism and Cardiometabolic Risk Factors (27/MN/2018)

Poznan University of Medical Sciences (PUMS)

Status

Completed

Conditions

Polymorphism, Restriction Fragment Length

Treatments

Genetic: gene expression

Study type

Observational

Funder types

Other

Identifiers

NCT04077554

359/15 & 1309/18

Details and patient eligibility

About

The aim of the study is to determine the relationship between VEGF gene expression, VEGF gene polymorphism and serum leptin concentration in the Polish population in people with excessive body weight. In addition, the aim of the study is to look for relationships between the VEGF gene polymorphism and anthropometric and biochemical factors of cardiovascular risk and endothelial dysfunction such as body weight, waist circumference, serum total cholesterol, LDL, HDL, triglycerides, glucose and the occurrence of cardiovascular diseases in the family of a patient with excessive body weight in the Polish population.

Full description

400 people (250 - study group; 150 control group) were subjected to subjective and objective action. Information on the occurrence of diseases and cardiovascular risk in the participant and his family was collected from research studies. Anthropometric parameters were measured (body weight, height, BMI, waist circumference, neck circumference) as well as blood pressure and pulse measurement. In addition, fasting venous blood was collected and secured. In venous blood currently marked with the following concentration: glucose, required cholesterol, LDL cholesterol, HDL cholesterol and triglycerides. In addition, a method of salting out and protecting DNA was developed from blood.

Furthermore the polymorphisms of VEGF genes (in positions: -2578 and -634) using the HMR (High Resolution Melt) method was determined.

Also VEGF and leptin by ELISA was determined. After obtaining the results of comparative analysis of the correlation between the occurrence of VEGF gene polymorphisms, serum levels of leptin and VEGF, and anthropometric and biochemical parameters of cardiovascular risk.

Enrollment

400 patients

Sex

All

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

informed consent in writing
stable body mass (+/- 1 kg)
excessive body mass (BMI ≥ 25 kg/m2- study group)
proper body mass (BMI < 25 kg/m2- control group)

Exclusion criteria

age <18 years
secondary obesity
pregnancy, lactation
other conditions that in the opinion of researchers may pose any risk to the patient during the study.