Reliability for the Detection of Sperm DNA Damage by a Sperm Chromatin Dispersion Test (Halosperm)

University Hospital, Clermont-Ferrand

Status: Completed

Conditions: Infertile Men

Treatments: Other: HALOSPERM

Study type: Observational

Funder types: Other

Identifiers

NCT02252510

CHU-0208

Details and patient eligibility

About

It is proposed to measure sperm DNA damage by sperm chromatin dispersion test. This measurement helps to better diagnose male infertility and seems easy to apply in routine. However, the reliability of this method needs to be evaluated before applying for clinical practice in andrology laboratory.

Full description

The aim of this study is to measure the inter and intra-observer reliability for the measurements of sperm DNA damage by sperm chromatin dispersion test (halosperm). The gold standard to measure sperm DNA damage is TUNEL (Terminal desoxynucleotidyl end labelling) method. However, this method is not easy to apply in routine. Therefore we investigate to analyse the inter-method reliability between sperm chromatin dispersion test and TUNEL.

Enrollment: 40 patients

Sex: Male

Ages: 18 to 50 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• infertile men
• <50 years
• with at least 5.106spermatozoa/ml in the ejacu

Exclusion criteria

• azoospermia