Renin Angiotensin Aldosterone System (RAAS) and Fibrinolysis in Humans: ACEi and PE5i

Vanderbilt University

Status: Completed

Conditions: Metabolic Syndrome

Treatments: Drug: placebo; Drug: Ramipril; Drug: Tadalafil

Study type: Interventional

Funder types: Other; NIH

Identifiers

NCT00750308

060198

Details and patient eligibility

About

This study will measure the effect of the agent tadalafil on glucose and insulin homeostasis in people with metabolic syndrome in the presence and absence of an ACE inhibitor.

Enrollment: 27 patients

Sex: All

Ages: 18 to 70 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

1. Ambulatory subjects, 18 to 70 years of age, inclusive

2. For female subjects, the following conditions must be met:

   • Postmenopausal status for at least 1 year,
   • Status-post surgical sterilization, or
   • If of childbearing potential, utilization of adequate birth control, and willingness to undergo urine beta-hcg testing prior to drug treatment and on every study day

3. Metabolic syndrome as defined by 3 or more of the following:

   • Fasting plasma glucose of at least 100 mg/dL (5.5 mmol/L),
   • Serum triglycerides of at least 150 mg/dL (1.7 mmol/L),
   • Serum HDL cholesterol less than 40 mg/dL (1.04 mmol/L) in males and less than 50mg/dl (1.30mmol/L) in females,
   • Blood pressure of at least 130/85 mmHg, or
   • Waist girth of more than 102 cm in men or 88 cm in women

Exclusion criteria

Subjects presenting with any of the following will not be included in the study:

1. Diabetes type 1 or type 2, as defined by a fasting glucose of 126 mg/dL or greater or the use of anti-diabetic medication
2. Use of hormone replacement therapy
3. Statin therapy
4. In hypertensive subjects, a seated systolic blood pressure greater than 179 mmHg or a seated diastolic blood pressure greater than 110 mmHg
5. Pregnancy
6. Breast-feeding
7. Cardiovascular disease such as myocardial infarction within 6 months prior to enrollment, presence of angina pectoris, significant arrhythmia, congestive heart failure (LV hypertrophy acceptable), deep vein thrombosis, pulmonary embolism, second or third degree heart block, mitral valve stenosis, aortic stenosis or hypertrophic cardiomyopathy
8. Treatment with anticoagulants
9. History of serious neurologic disease such as cerebral hemorrhage, stroke, or transient ischemic attack
10. History or presence of immunological or hematological disorders
11. Diagnosis of asthma
12. Clinically significant gastrointestinal impairment that could interfere with drug absorption
13. Impaired hepatic function (aspartate amino transaminase [AST] and/or alanine amino transaminase [ALT] > 1.5 x upper limit of normal range)
14. Impaired renal function (serum creatinine > 1.5 mg/dl)
15. Hematocrit < 35%
16. Any underlying or acute disease requiring regular medication which could possibly pose a threat to the subject or make implementation of the protocol or interpretation of the study results difficult
17. Treatment with chronic systemic glucocorticoid therapy (more than 7 consecutive days in 1 month)
18. Treatment with lithium salts
19. History of alcohol or drug abuse
20. Treatment with any investigational drug in the 1 month preceding the study
21. Mental conditions rendering the subject unable to understand the nature, scope and possible consequences of the study
22. Inability to comply with the protocol, e.g. uncooperative attitude, inability to return for follow-up visits, and unlikelihood of completing the study

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: Quadruple Blind

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27 participants in 12 patient groups

tadalafil, ramapril, combo, placebo

Active Comparator group

Description:

placebo+tadalafil for three weeks, washout, placebo+ramipril for three weeks, washout, ramipril + tadalafil, washout, placebo+placebo for three weeks

Treatment:

Drug: Tadalafil

Drug: Ramipril

Drug: placebo

Drug: placebo

ramipril, tadalafil, placebo, combo

Active Comparator group

Description:

placebo+ramipril for three weeks, washout, placebo+tadalafil for three weeks, washout, placebo+placebo for three weeks, washout, ramipril+tadalafil for three weeks

Treatment:

Drug: Tadalafil

Drug: Ramipril

Drug: placebo

Drug: placebo

combo, placebo, tadalafil, ramipril

Active Comparator group

Description:

ramipril+tadalafil for three weeks, washout, placebo+placebo for three weeks, washout, placebo+tadalafil for three weeks, washout, placebo+ramipril for three weeks

Treatment:

Drug: Tadalafil

Drug: Ramipril

Drug: placebo

Drug: placebo

placebo, combo, ramipril, tadalafil

Active Comparator group

Description:

placebo+placebo for three weeks, washout, ramipril+tadalafil for three weeks, washout placebo+ramipril for three weeks, washout, placebo+tadalafil for three weeks

Treatment:

Drug: Tadalafil

Drug: Ramipril

Drug: placebo

Drug: placebo

tadalafil, placebo, ramipril, combo

Active Comparator group

Description:

placebo+tadalafil for three weeks, washout, placebo+placebo for three weeks, washout, placebo+ramipril for three weeks, washout, ramipril+tadalafil for three weeks

Treatment:

Drug: Tadalafil

Drug: Ramipril

Drug: placebo

Drug: placebo

ramipril, combo, tadalfil, placebo

Active Comparator group

Description:

placebo+ramipril for three weeks, washout, ramipril+tadalafil for three weeks, washout, placebo+tadalafil for three weeks, washout, placebo for three weeks

Treatment:

Drug: Tadalafil

Drug: Ramipril

Drug: placebo

Drug: placebo

combo, ramipril, placebo, tadalafil

Active Comparator group

Description:

ramipril+tadalafil for three weeks, washout, placebo+ramipril for three weeks, washout, placebo+placebo for three weeks, washout, placebo+tadalafil for three weeks

Treatment:

Drug: Tadalafil

Drug: Ramipril

Drug: placebo

Drug: placebo

placebo, tadalafil, combo, ramipril

Active Comparator group

Description:

placebo+placebo for three weeks, washout, placebo+tadalafil for three weeks, washout, ramipril+tadalafil for three weeks, washout, placebo+ramipril for three weeks

Treatment:

Drug: Tadalafil

Drug: Ramipril

Drug: placebo

Drug: placebo

tadalafil, combo, placebo, ramipril

Active Comparator group

Description:

placebo+tadalafil for three weeks, washout, ramipril+tadalafil for three weeks, washout, placebo+placebo for three weeks, washout, placebo+ramipril for three weeks

Treatment:

Drug: Tadalafil

Drug: Ramipril

Drug: placebo

Drug: placebo

ramipril, placebo, combo, tadalafil

Active Comparator group

Description:

placebo+ramipril for three weeks, washout, placebo+placebo for three weeks, washout, ramipril+tadalafil for three weeks, washout, placebo+tadalafil for three weeks

Treatment:

Drug: Tadalafil

Drug: Ramipril

Drug: placebo

Drug: placebo

combo, tadalafil, ramipril, placebo

Active Comparator group

Description:

ramipril+tadalafil for three weeks, washout, placebo+tadalafil for three weeks, washout, placebo+ramipril for three weeks, washout, placebo+placebo for three weeks

Treatment:

Drug: Tadalafil

Drug: Ramipril

Drug: placebo

Drug: placebo

placebo, ramipril, tadalafil, combo

Active Comparator group

Description:

placebo+placebo for three weeks, washout, placebo+ramipril for three weeks, washout, placebo+tadalafil for three weeks, washout, ramipril+tadalafil for three weeks

Treatment:

Drug: Tadalafil

Drug: Ramipril

Drug: placebo

Drug: placebo