Reparixin in Prevention of Delayed Graft Function After Kidney Transplantation

Dompé

Status and phase: Completed; Phase 2

Conditions: Kidney Diseases; Ischemia-Reperfusion Injury

Treatments: Drug: reparixin intermittent infusion; Other: placebo infusion; Drug: Reparixin continuous infusion

Study type: Interventional

Funder types: Industry

Identifiers

NCT00248040

REP0204

Details and patient eligibility

About

The chemokine CXCL8 plays a key role in the recruitment and activation of polymorphonuclear neutrophils in post-ischemia reperfusion injury after solid organ transplantation. Reparixin is a novel, specific inhibitor of CXCL8. This study is configured to explore the safety and efficacy of reparixin in preventing the delayed graft function (DGF) after kidney transplantation.

Full description

Delayed graft function (DGF) is the most common allograft complication in the immediate kidney post-transplant period, affecting 25-35% of all patients who receive a cadaver graft, but rates up to 50% have been reported, especially in recipients of kidneys from marginal donors. It is an important clinical complication as it requires dialysis, prolongs hospitalisation, raises the cost of transplantation, and makes more difficult the management of immunosuppressive therapy. Although the effects of DGF on long-term graft function are still debated, there is overall increasing evidence that DGF reduces long-term graft survival. Moreover, given the well documented impact of acute rejection on long-term graft survival, it is conceivable that DGF and acute rejection synergize in negatively influencing long-term graft survival. Kidney reperfusion, after long cold ischemia period, is associated with an inflammatory reaction characterized by massive polymorphonuclear leukocyte (PMN) infiltration both at the glomerular and tubular levels. The importance of CXCL8 in recruiting PMN in kidney tissue during the ischemic time and after reperfusion has been clearly documented.

The efficacy of reparixin in preventing PMN infiltration and tissue damage in rat models of kidney transplantation and lung transplantation, as well as the safety shown in human phase 1 studies, provide the rationale for a clinical study aimed at evaluating the effect of reparixin in preventing DGF after kidney transplantation

Enrollment: 80 patients

Sex: All

Ages: 18 to 65 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Male and female patients accepted and listed for renal transplantation due to end stage renal disease (ESRD)
• Planned isolated single kidney transplant from a non-living donor with brain death
• Recipients of a kidney maintained in cold storage
• Recipients at risk of developing DGF
• Planned induction with steroids + mycophenolate mofetil (MMF) or mycophenolic acid + biological induction
• Patient willing and able to comply with the protocol procedures for the duration of the study, including scheduled follow-up visits and examinations
• Patient given written informed consent, prior to any study-related procedure not part of normal medical care, with the understanding that consent may be withdrawn by the patient at any time without prejudice to their future medical care

Exclusion criteria

• Recipients of an intended multiple organ transplant
• Recipients of a kidney from a living donor
• Recipients of a kidney from a non-heart beating donor
• Recipients of double kidney transplant
• Re-transplant >2
• Recipients of a kidney maintained by pulsatile machine perfusion
• Concurrent sepsis
• Recipients with hepatic dysfunction at the time of transplant
• Clinical contraindications to central line access, or arteriovenous fistula, if any, not suitable for infusion of investigational product
• Hypersensitivity to non steroidal anti-inflammatory drugs (NSAIDs)
• Patients simultaneously participating in any other clinical trials involving an investigational drug not yet authorized for use in kidney transplant
• Pregnant or breast-feeding women

Trial design

Primary purpose: Prevention

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Double Blind

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80 participants in 3 patient groups, including a placebo group

reparixin group - continuous infusion

Experimental group

Description:

Continuous iv infusion into a (high flow) central vein (or through an arterio-venous fistula) by an infusion pump. A dose of 2.772 mg/kg/h was administered for12 hours.

Treatment:

Drug: Reparixin continuous infusion

reparixin group - intermittent infusion

Experimental group

Description:

Intermittent iv infusion into a (high flow) central vein (or through an arterio-venous fistula) by an infusion pump. A dose of 2.244 mg/kg was administered over a 30-minute period, followed by a 1.5-hour interval. Twelve doses were administered over a total period of 22.5 hours.

Treatment:

Drug: reparixin intermittent infusion

placebo infusion

Placebo Comparator group

Description:

Continuous/intermittent iv infusion of a volume/schedule matched saline into a (high flow) central vein (or through an arterio-venous fistula) by an infusion pump.

Treatment:

Other: placebo infusion