Repeated Injections of i-PRF for Decreasing Root Resorption After the Orthodontic Treatment.

Damascus University

Status: Completed

Conditions: Crowding of Anterior Maxillary Teeth

Treatments: Procedure: 4-time i-PRF injection group; Procedure: Saline injection

Study type: Interventional

Funder types: Other

Identifiers

NCT07352241

UDDS-Ortho-1-2026

Details and patient eligibility

About

Mandibular incisor crowding is a frequent issue that prompts many adults to seek orthodontic treatment. The treatment may enhance patients' quality of life, facilitate dental plaque control, and improve the quality of periodontal tissue. However, this treatment is accompanied by several side effects, including resorption of the apical portion of the teeth. Platelet-rich concentrates, such as injectable platelet-rich fibrin, have been shown to have positive effects on both soft- and hard-tissue healing. They may serve as a beneficial self-derived material to prevent this complication.

Full description

In recent years, increasing attention has been directed toward biologically driven approaches to modulate tissue responses during orthodontic treatment and minimize adverse effects, such as root resorption. Platelet-rich fibrin (PRF), a second-generation autologous platelet concentrate, has emerged as a promising biomaterial due to its high concentration of platelets, leukocytes, and a fibrin matrix that allows gradual release of multiple growth factors, including platelet-derived growth factor (PDGF), transforming growth factor-β (TGF-β), vascular endothelial growth factor (VEGF), and insulin-like growth factor (IGF). However, in 2014, injectable platelet-rich fibrin (I-PRF), which is similar to PRF, was introduced in France by Choukroun, using non-glass tubes and adjusting the centrifugal force. Just like traditional PRF, i-PRF contains high levels of growth factors, stem cells, leukocytes, and cytokines.

These growth factors play a crucial role in angiogenesis, tissue regeneration, modulation of inflammation, and bone remodeling, all of which are central processes in orthodontic tooth movement and periodontal healing.

Enrollment: 36 patients

Sex: All

Ages: 17 to 25 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Adult patients aged between 17 and 28 years
• Class I malocclusion with moderate crowding (4-6 mm) according to Little's Irregularity Index (LII)
• Normal or inclination for mandibular incisors
• Complete permanent teeth.

Exclusion criteria

• Previous orthodontic treatment,
• Poor oral hygiene,
• Severe skeletal discrepancy,
• The presence of coagulation disorders or being under anticoagulant treatment.

Trial design

Primary purpose: Treatment

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Single Blind

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36 participants in 2 patient groups, including a placebo group

4-time i-PRF injection group

Experimental group

Description:

I-PRF was injected in the buccal region of the mandibular incisors 4 sessions with 28-day interval.

Treatment:

Procedure: 4-time i-PRF injection group

Control group

Placebo Comparator group

Description:

Saline serum was injected in the buccal region of the mandibular incisors 4 sessions with 28-day interval

Treatment:

Procedure: Saline injection