Resistance Exercise and Hydrolyzed Collagen Supplementation

Rob Erskine

Status: Completed

Conditions: Diet, Food, and Nutrition

Treatments: Dietary Supplement: Experimental: Consumption of two different doses of hydrolyzed collagen (HC) with reistance exercise; Dietary Supplement: Experimental: Consumption of three different doses of hydrolyzed collagen (HC) with reistance exercise

Study type: Interventional

Funder types: Other

Identifiers

NCT05932771

18/SPS/059

Details and patient eligibility

About

This study aimed to investigate the effect of different doses of hydrolyzed collagen (HC) with resistance exercise (RE) on whole body collagen synthesis in healthy young and older males and females.

Full description

This study aimed to investigate the effect of different doses of hydrolyzed collagen (HC) with resistance exercise (RE) on whole body collagen synthesis in healthy young and older males and females.

Healthy young and older males and females ingested 0 grams, 15 grams or 30 grams HC with 50 milligrams vitamin C 1h prior to performing four sets' barbell back-squat RE at 10-repetition maximum load, after which they rested for six hours. Blood samples were collected throughout each trial to analyse procollagen type Ⅰ N-terminal propeptide (PⅠNP, a biomarker of collagen synthesis) and β-isomerized C-terminal telopeptide of type I collagen (β-CTX, a biomarker of collagen breakdown) concentration, and the concentration of 18 amino acids that constitute collagen.

This is the first study to investigate the combined effect of different doses HC with high-intensity RE on whole body collagen synthesis in healthy young and older men and women. If 30 grams HC intake with RE does augment collagen synthesis more than RE alone, this suggests that long-term HC intake with chronic RE would be beneficial for tendon health, because collagen is the most abundant protein in tendon.

Enrollment: 21 patients

Sex: All

Ages: 18 to 65 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• At least 12 months experienced in resistance exercise (regular back squat experience)
• Free from musculoskeletal injury
• Nullipara (a woman who has never given birth) (only for young female participatns)

Exclusion criteria

• Having a history of patellar tendon pathology
• Vegan
• Consumed nutritional supplements or medication purported to have beneficial effects on muscle-tendon properties (e.g. antioxidants, protein, etc.)
• Had a sustained a lower limb injury in the previous six months
• Smoker/vaper
• <18 or >40 years old (only for young participants)
• <40 ro >65 years old (only for older participants)
• Having an irregular menstrual cycle (only for young female participants)

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Crossover Assignment

Masking: Triple Blind

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21 participants in 4 patient groups

1.Trial for young male participants: Consuming hydrolyzed collagen (HC) with reistance exercise

Experimental group

Description:

Young male participants consumed one of three different HC doses (0 grams, 15 grams, or 30 grams) with 4 sets of 10 repetitions of barbell back squat exercise at 10-repetition maximum load in a random order and a seven-day wash-out period interspersed between each trial.

Treatment:

Dietary Supplement: Experimental: Consumption of three different doses of hydrolyzed collagen (HC) with reistance exercise

2.Trial for young female participant: Consuming hydrolyzed collagen (HC) with reistance exercise

Experimental group

Description:

The intervention procedure is exactly same as Arm 1 except for the number of visits. Young female participant was asked to visit the laboratory on four occasions during two consecutive months. Therefore, there were two trials in each month, where female participants' estrogen level was lower (i.e., onset of menses) or higher (i.e., ovulation). Dates for the trials were determined based on self-report of onset of menses and previous menstrual cycle length.

Treatment:

Dietary Supplement: Experimental: Consumption of two different doses of hydrolyzed collagen (HC) with reistance exercise

3.Trial for older male participants: Consuming hydrolyzed collagen (HC) with reistance exercise

Experimental group

Description:

The age range of older male participants was 40 - 65 years. The intervention procedure is exactly same as Arm 1

Treatment:

Dietary Supplement: Experimental: Consumption of three different doses of hydrolyzed collagen (HC) with reistance exercise

4.Trial for older female participants: Consuming hydrolyzed collagen (HC) with reistance exercise

Experimental group

Description:

The age range of older female participants was 40 - 65 years. The intervention procedure is exactly same as Arm 2

Treatment:

Dietary Supplement: Experimental: Consumption of two different doses of hydrolyzed collagen (HC) with reistance exercise