Role of IL 12 and IL 23 as Potential Biomarkers in Diagnosis of Early Crohn's Disease

Tanta University

Status: Completed

Conditions: Interleukin 23; Diagnosis; Biomarker; Interleukin 12; Crohn Disease

Treatments: Procedure: Immunohistochemistry procedure

Study type: Observational

Funder types: Other

Identifiers

NCT06844565

36264PR1059/1/25

Details and patient eligibility

About

This study aims to evaluate the role of Interleukin (IL)-12 and IL-23 as potential biomarkers in the diagnosis of early Crohn's disease.

Full description

Crohn's disease (CD) is characterized by periods of remission and relapse, with symptoms ranging from abdominal pain and diarrhea to severe complications such as fistulas and intestinal obstruction.

Interleukins (ILs) are known clusters of cytokines that regulate the proliferation, development, and activation of immune cells. IL-12 is predominantly a pro-inflammatory cytokine secreted by antigen-presenting cells (APC) in response to sensing of microbial components by Toll-like receptors. Another member of this family, IL-23 is also produced by DCs and macrophages after toll-like receptors (TLRs) engagement.

Enrollment: 110 patients

Sex: All

Ages: 18 to 65 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Age from 18 to 65 years.
• Both sexes.
• Patients who are suspected of having Crohn's disease clinically and by colonoscopy (like ulcers, fistulas, or skip lesions).
• Non-specific ileitis by histopathology.

Exclusion criteria

• Patients with no pathological samples through colonoscopy.
• History of other significant gastrointestinal diseases (e.g., colon cancer, ischemic bowel disease).
• Immunosuppressive therapy or biologic agents at the time of study.
• Previous abdominal surgeries.

Trial design

110 participants in 1 patient group

Study group

Description:

Patients who are suspected of having Crohn's disease clinically and by colonoscopy (like ulcers, fistulas, or skip lesions). Non-specific ileitis by histopathology.

Treatment:

Procedure: Immunohistochemistry procedure