Role of Regulatory B Cells in Behçet's Disease

Assiut University

Status: Completed

Conditions: Behcet Disease

Treatments: Diagnostic Test: 2D Echocardiographic analysis; Diagnostic Test: Two-Dimensional speckle tracking echocardiography; Diagnostic Test: Flow-cytometric assay

Study type: Observational

Funder types: Other

Identifiers

NCT04376411

17300388

Details and patient eligibility

About

There are limited data about the role of regulatory B cells in Behcet Disease. In this study we aimed to identify the proportions of total B lymphocytes and their regulatory subset in different Behcet Disease phenotype and therapies concentrating on the cardiovascular system attempting to unravel their function in Behcet Disease.

Full description

Behçet's disease is a chronic multi-organ vasculitis characterized by recurrent oral and genital aphthae, skin lesions, widespread thrombosis and aneurysmal formation, and serious involvement of the eyes and central nervous system.

The innate and adaptive immune cells integrate in the pathogenesis of Behçet's disease. Interestingly, the gene expression analysis of Behçet's disease patients and healthy controls revealed the modulation of a range of genes involved in biological processes in Behçet's disease such as inflammation, apoptosis, angiogenesis, blood coagulation, vascular damage, signalling pathways, and immune responses, particularly, in innate immune cells, Th17 cells and B cells. Yet, the regulatory role of B cells in Behçet's disease has not been thoroughly investigated. Therefore, the aim of the current study is to identify the proportions of total B lymphocytes and their regulatory subset in different Behçet's disease phenotypes and therapies attempting to unravel their function in Behcet Disease. The role of B cells and regulatory B cells in the cardiovascular system affection in Behcet Disease was studied in more details.

Enrollment: 74 patients

Sex: All

Ages: 18 to 60 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Thirty five adult patients who fulfilled the diagnostic criteria of Behcet Disease, and 39 age and sex matched healthy subjects as control

Exclusion criteria

• Patients younger than 18 years old and those who were affected by other rheumatic disease other than Behcet Disease.

Trial design

74 participants in 2 patient groups

patients with Behcet disease

Description:

Flow-cytometric assay Detailed 2 Di mention Echocardiographic analysis Two-Dimensional speckle tracking echocardiography

Treatment:

Diagnostic Test: 2D Echocardiographic analysis

Diagnostic Test: Two-Dimensional speckle tracking echocardiography

Diagnostic Test: Flow-cytometric assay

Healthy control subjects

Description:

Flow-cytometric assay

Treatment:

Diagnostic Test: Flow-cytometric assay