Role of T Follicular Helper Cells in Autoimmune Hemolytic Anemia (TFH in AIHA) (TFH and AIHA)

University Hospital Center (CHU) Dijon Bourgogne

Status

Completed

Conditions

Autoimmune Hemolytic Anemia

Treatments

Procedure: spleen sample

Biological: blood sample

Study type

Observational

Funder types

Other

Identifiers

NCT02828670

AUDIA APJ 2014

Details and patient eligibility

About

T follicular helper (TFH) cells represent a T cell subset dedicated to the activation of B cells. They have been involved in the pathogenesis of autoimmune diseases in humans such as lupus and Sjögren disease. We recently showed that TFH are implicated in the activation of autoreactive B lymphocytes during ITP. Autoimmune hemolytic anemia (AIHA) is an autoimmune disease due to antibodies targeting red blood cells. To date, the role of TFH in the pathogenesis of AIHA is not known.

We hypothesize that AIHA is associated with an increase in the number and/or function of TFH, that could participate in the activation of autoreactive B lymphocytes.

Enrollment

25 patients

Sex

All

Ages

18+ years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

Patients with a diagnosis of warm AIHA defined by
- Hemoglobin <11 g/dl
- Low haptoglobin level
- Positive direct antiglobulin test (IgG or IgG + complement)
- Reticulocyte count >120 G/L
Patients
- naive to treatment for hemolytic anemia or a in relapse
- Older than 18 years
- Able to understand written and spoken French
Persons who have provided written consent

Control population

Patients who have given consent
Patients over 18 years
Patients with cold agglutinin disease (progressive AIHA and different treatments of warm autoantibody AIHA) can be included as a sub-group of controls
Patients with non-auto-immune hemolytic anemia (constitutional of enzymatic origin, membrane-related,...) can be included as a sub-group of controls

Exclusion criteria