Role of the NLRP3 Inflammasome in Escherichia Coli and Staphylococcus Aureus Bacteria (NLRP3-Bact)

Centre Hospitalier Universitaire de Nice

Status: Terminated

Conditions: Escherichia Coli Infections; Staphylococcus Aureus

Treatments: Genetic: Sample analysis

Study type: Observational

Funder types: Other

Identifiers

NCT03869593

18-AOI-08

Details and patient eligibility

About

Our previous studies delineate a novel pathway of immune activation in animals that the investigators have named Anti-Virulence Immunity (AVI). Using a mice model of bacteremia, the investigators have demonstrated that Escherichia coli Cytotoxic Necrotizing Factor 1 (CNF1) activity is sensed by the immune system. This immune sensing results in a rapid bacterial clearing during bacteremia triggered by uropathogenic E. coli-expressing CNF1. The investigators already confirmed the involvement of one inflammasome using macrophages isolated from Knock-out mice. The investigators have recently determined the conservation in human monocytes of the interleukin -1beta maturation triggered by CNF1 and observed the heterogeneous capacity of monocytes to respond to the CNF1 treatment depending on the donors. Here, to determine the importance in natura of AVI the investigators will analyze the blood content of patients presenting E. coli and S. aureus bacteremia. The DNA of monocytes isolated from patients will be extracted and various genes implicated in the activity of various inflammasomes will be sequenced to identify mutations that could explain the susceptibility to bacteremia or a specific clinical presentation, i.e. requirement of a management in ICU because of organ failure.

Enrollment: 60 patients

Sex: All

Ages: 8 to 75 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Patient with S. aureus or E. coli bacteriostatic bacteremia defined by at least one positive blood culture bottle
• Patient requiring a blood test as part of his bacteremia
• not subject to a judicial protection measure
• Signature of the non-opposition of consent (for minor patients signed by one of the parents or the representative of the parental authority)
• Affiliation to social security

Exclusion criteria

• Immunocompromised patient defined by:

  • current immunosuppressive therapies: corticosteroids, chemotherapy, biotherapy
  • solid organ transplant patient or hematopoietic stem cell transplant
  • chemotherapy-induced neutropenia
  • Congenital immune deficiency

• bacteremia related to a peripheral or central catheter

• Urinary obstruction not lifted within the first 24 hours of management

• Intra-abdominal infection collected undrained in the first 24 hours of management

• primary infectious focus represented by mechanically ventilated pneumonia

• Pregnant or lactating woman

Trial design

60 participants in 1 patient group

Patients presenting E. coli and S. aureus bacteremia

Description:

Here, to determine the importance of the mutation we will analyze the blood content of patients presenting E. coli and S. aureus bacteremia

Treatment:

Genetic: Sample analysis