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Safety and Immune Response to FMPV-1

H

Hubro Therapeutics

Status and phase

Completed
Phase 1

Conditions

Colorectal Cancer

Treatments

Biological: GM-CSF (as adjuvant)
Biological: FMPV-1

Study type

Interventional

Funder types

Industry

Identifiers

NCT05238558
FMPV-1-01
QSC204718 (Other Identifier)

Details and patient eligibility

About

This is a single centre, open-label, non-randomized, Phase I study assessing safety and immune response of FMPV-1 in healthy male subjects.

Full description

To date, there is no clinical experience with FMPV-1. This is the first clinical study with FMPV-1 in healthy volunteers to assess the safety and immunogenicity of FMPV-1 in man. The study population will include 16 healthy males in 2 cohorts (8 per cohort) to ensure data in a minimum of 14 subjects.

Up to two dose levels of FMPV-1 in conjunction with the adjuvant granulocyte macrophage colony stimulating factor (GM-CSF) will be assessed - the dose in Cohort 2 will depend on the safety and specific delayed type hypersensitivity (DTH) response data from Cohort 1. Each cohort will follow the same study design. Following preliminary screening procedures, subjects will be admitted in the morning on the day before initial dosing (Day -1). In Cohorts 1 and 2, subjects will receive FMPV-1 15 ± 5 min after GM-CSF on Days 1, 8, 15, 29 and 43 as an intradermal injection into the back of the upper arm. In Cohort 1, 8 subjects will receive GM-CSF (0.03 mg) + FMPV-1 (0.15 mg/injection: low dose) administered as 2 separate intradermal injections. In Cohort 2, 8 subjects will receive GM-CSF (0.03 mg) + FMPV-1 (either 0.15 or 0.3 mg/injection - dependent on Cohort 1 data) administered as 2 separate intradermal injections.

Sentinel dosing will be applied for both cohorts if different doses are used; if the same dose is to be used in both cohorts, sentinel dosing will not be applied in Cohort 2.

Adverse events and laboratory assessments will be the endpoints to assess the safety of GM-CSF/FMPV-1 vaccination. FMPV-1 specific DTH responses and FMPV-1-specific proliferative T-cell responses will be the endpoints to assess the FMPV-1 specific immune response. Subjects will receive a total of 8 administrations intradermally: GM-CSF + FMPV-1 on 5 separate occasions and FMPV-1 (without GM-CSF) on 3 occasions for the DTH skin reactivity assessment. The main part of the study will last for approximately 16 weeks, including screening, and subjects will return for follow-up visits after 6 months and 12 months. The overall estimated time from screening until the final follow-up visit is approximately 12 months. All adverse reactions will be collected until early withdrawal or discharge from the study after the 12-month follow-up visit.

Enrollment

16 patients

Sex

Male

Ages

18 to 55 years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

  1. Healthy males
  2. Aged 18 to 55 years inclusive at the time of signing informed consent
  3. Body mass index (BMI) of 18.0 to 32.0 kg/m2 as measured at screening
  4. Must provide written informed consent
  5. Must agree to adhere to the contraception requirements: male subjects who are sexually active with a partner of childbearing potential must use a condom plus an approved method of highly effective contraception from the time of informed consent until 90 days (one cycle of spermatogenesis) after last vaccine administration.

Exclusion criteria

  1. Subjects who have received any investigational medicinal product in a clinical research study within the 90 days prior to Day 1 or within less than 5 elimination half-lives prior to Day 1, whichever is longer

  2. Subjects who are, or are immediate family members of, a study site or sponsor employee

  3. Subjects who have previously been administered investigational medicinal product in this study.

  4. Evidence of current SARS-CoV-2 infection

  5. History of any drug or alcohol abuse in the past 2 years

  6. Regular alcohol consumption defined as >21 units per week (1 unit = ½ pint beer, or a 25 mL shot of 40% spirit, 1.5 to 2 units = 125 mL glass of wine, depending on type)

  7. A confirmed positive alcohol breath test at screening or admission

  8. Current smokers and those who have smoked within the last 6 months. A confirmed breath carbon monoxide reading of greater than 10 ppm at screening or admission

  9. Current users of e-cigarettes and nicotine replacement products and those who have used these products within the last 6 months

  10. Subjects with pregnant or lactating partners

  11. Clinically significant abnormal clinical chemistry, haematology, coagulation or urinalysis as judged by the investigator. Subjects with Gilbert's Syndrome are not allowed.

  12. Confirmed positive drugs of abuse test result

  13. Positive hepatitis B surface antigen (HBsAg), hepatitis C virus antibody (HCV Ab) or human immunodeficiency virus (HIV) antibody results

  14. History of clinically significant cardiovascular, renal, hepatic, dermatological, chronic respiratory or gastrointestinal disease, neurological or psychiatric disorder, as judged by the investigator

  15. Serious adverse reaction or serious hypersensitivity to any drug or the formulation excipients

  16. Presence or history of a clinically significant allergy requiring treatment, as judged by the investigator. Hay fever is allowed unless it is active

  17. Subjects currently receiving any agent with a known effect on the immune system within 90 days before first investigational medicinal product administration

  18. Donation of blood or plasma within the previous 3 months or loss of greater than 400 mL of blood

  19. Subjects who are taking, or have taken, any prescribed or over-the-counter medications, herbal remedies or antihistamines (other than vitamin supplements and/or up to 4 g of paracetamol per day) in the 7 days before investigational medicinal product administration

  20. Subjects who have had a vaccine (including COVID-19 vaccine) within 28 days before first dose

  21. Subjects with tattoos or scars on the arms which may interfere with injection site assessments, as determined by the investigator or delegate at screening

  22. Subjects with any other illnesses or medical conditions such as, but not limited to:

    • Any infection that requires systemic treatment (any grade)
    • Cardiac failure (any grade)
    • Systemic and gastrointestinal inflammatory conditions
    • Bone marrow dysplasia
    • History of auto-immune disease
    • History of adverse reactions to any vaccines
    • History of adverse reactions to GM-CSF
  23. Subjects with any other malignancies within the last 3 years (except for adequately treated carcinoma of the cervix or basal or squamous cell skin cancer)

  24. Subjects planning to receive a yellow fever or other live (attenuated) vaccine during the course of study

  25. Subject has a first degree relative with a haematological malignancy

  26. Failure to satisfy the investigator of fitness to participate for any other reason

Trial design

Primary purpose

Treatment

Allocation

N/A

Interventional model

Single Group Assignment

Masking

None (Open label)

16 participants in 1 patient group

FMPV-1 vaccination
Experimental group
Description:
GM-CSF (0.03 mg) + FMPV-1 (0.15 mg/injection: low dose) administered as 2 separate intradermal injections. Total of 8 administrations intradermally; GM-CSF + FMPV-1 on 5 separate occasions and FMPV-1 (without GM-CSF) on 3 occasions for the DTH skin reactivity assessment.
Treatment:
Biological: FMPV-1
Biological: GM-CSF (as adjuvant)

Trial contacts and locations

1

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Data sourced from clinicaltrials.gov

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