Senescent Endometrial Cells in Patients with Thin Endometrial Lining

Trio Fertility

Status: Enrolling

Conditions: Thin Endometrial Lining; Cell Senescence; Asherman's Syndrome

Treatments: Procedure: Endometrial Biopsy

Study type: Observational

Funder types: Other

Identifiers

NCT06450301

3533

Details and patient eligibility

About

The goal of this observational study is to determine if thin endometrial lining that is unresponsive to estrogen might be associated with the presence of senescent cells in patients following long-term use of oral contraceptives. The main question it aims to answer is:

Are there any senescent cells present in thin endometrial lining?

During the luteal phase of the cycle, participants will will undergo:

• Gynecologic US to measure the endometrial lining thickness and pattern.
• Endometrial biopsy with Pipelle catheter

Full description

The investigators hypothesized that senescent cells in the endometrium may be the cause of the failure of thin endometrium to respond to normal growth stimuli such as estrogen.

The outcome that research team anticipate to measure is the percentage of senescent cells in the endometrial biopsies of women with thin endometrium compared to the control group biopsies.

Enrollment: 100 estimated patients

Sex: Female

Ages: 18 to 39 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Age 18-39
• BMI - up to 35
• Thin endometrium after OCP use
• Asherman's syndrome
• Women already undergoing endometrial biopsy for infertility testing (eg. EMMA testing)
• Women with archived endometrial biopsy samples

Exclusion criteria

• Women with systemic disease (Rheumatic disease, DM)
• Women with coagulopathies
• Women with active pelvic inflammatory process
• Uterine anomalies
• Pregnant women
• Any hormonal contraception use or IUD

Trial design

100 participants in 3 patient groups

Thin endometrial lining

Description:

Patients following long-term use of oral contraceptives with known thin endometrial lining (less than 7mm, confirmed in at least two cycles and unresponsive to estrogen-induced growth). We will ask each woman to undergo an endometrial biopsy as part of the study during their luteal phase.

Treatment:

Procedure: Endometrial Biopsy

Asherman's syndrome

Description:

Patients with Asherman's syndrome following Dilation and curettage (D\&C). We will ask each woman to undergo an endometrial biopsy as part of the study during their luteal phase.

Treatment:

Procedure: Endometrial Biopsy

Control

Description:

Patients already scheduled to undergo an endometrial biopsy for infertility management. We will ask each woman to undergo an endometrial biopsy as part of the study during their luteal phase. In some cases, we may be able to access archived endometrial samples from women in the control group who agree to participate and who have already had an endometrial biopsy.

Treatment:

Procedure: Endometrial Biopsy