Sepsis and Thiol-Disulfide Balance in Emergency Department

Sakarya University

Status: Completed

Conditions: Oxidative Stress; Sepsis

Treatments: Diagnostic Test: Thiol- Disulfide Blood Sample Measurement

Study type: Interventional

Funder types: Other

Identifiers

NCT07134868

SAU-ED-MA-01

Details and patient eligibility

About

The aim of this study was to determine the effectiveness of thiol-disulfide homeostasis in predicting diagnosis and mortality in sepsis patients, and to compare it with other inflammatory markers.

Full description

Sepsis is a serious illness with high mortality rates triggered by infection-associated pathogenesis involving oxidative stress dysfunction. This study aims to elucidate the diagnostic efficacy of changes in thiol-disulfide homeostasis, its predictive role in mortality, relationship with other inflammatory parameters in sepsis patients in emergency department.

In prospective, randomized controlled study, 50 patients diagnosed with sepsis according to the Sepsis-3 guidelines who presented emergency department were compared with a control group of 50 healthy volunteers. Thiol-disulfide homeostasis parameters, including total thiol, native thiol, disulfide levels, reduced thiol, oxidized thiol, and thiol oxidation-reduction ratios, were examined. Additionally, inflammatory blood parameters (C-reactive protein, procalcitonin, lactate) and mortality durations were compared with thiol-disulfide homeostasis.

The objective of this study was to examine the effectiveness of thiol-disulfide homeostasis in forecasting diagnosis and mortality in sepsis patients, and to compare it against other inflammatory markers.

Enrollment: 100 patients

Sex: All

Ages: 18+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Patients aged 18 and above who met the diagnostic criteria were included in the study

Exclusion criteria

• Who did not meet the criteria for sepsis diagnosis according to the quick Sequential Organ Failure Assessment score
• Individuals with malignancy, acute coronary syndrome, acute cerebrovascular event, acute/chronic kidney/liver failure, rheumatologic diseases, febrile neutropenia, immunosuppressive disorders, corticosteroid use, and those
• Individuals with a history of antibiotic use within the last week

Trial design

Primary purpose: Diagnostic

Allocation: Non-Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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100 participants in 2 patient groups

Sepsis Group

Active Comparator group

Description:

Consisted of patients diagnosed with sepsis (defined as those presenting to the emergency department with suspected infection and scoring two or higher on the quick Sequential Organ Failure Assessment scoring system according to the Sepsis-3 guidelines) and who agreed to participate in the study.

Treatment:

Diagnostic Test: Thiol- Disulfide Blood Sample Measurement

Control Group

No Intervention group

Description:

Comprised of healthy volunteers who presented to the emergency department for any reason and agreed to participate in the study.