Sequential Versus Single O2 Levels on Human Embryo Development

Ibn Sina Hospital

Status: Completed

Conditions: Embryo Culture

Treatments: Other: Sequential O2 Culture

Study type: Interventional

Funder types: Other

Identifiers

NCT03630900

Ibnsina IVF-Oxygen

Details and patient eligibility

About

Myriads of elements affect human embryo culture in vitro. These include, but not limited to, air quality, culture media, culture temperature, incubator humidity, pH, and O2 levels. O2 tension has been thoroughly investigated and, to date, 5% O2 level is superior to 20%. Reports showed that lowering the O2 from Day 3 of embryo culture seems to be physiological.

Enrollment: 487 patients

Sex: Female

Ages: 18 to 40 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• ICSI cycles undergoing their first ICSI attempt

Exclusion criteria

• ICSI cycles with a previous failure

Trial design

Primary purpose: Other

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Double Blind

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487 participants in 2 patient groups

Sequential O2 culture (5% then 2.5%)

Experimental group

Description:

Embryo culture from day 0 to 3 in 5% O2 then split to either 5% or 2.5% O2 until Day 5 or 6.

Treatment:

Other: Sequential O2 Culture

5% O2 culture

No Intervention group