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Sequestration of Platelets Prior to Bypass Reduces Bleeding After Cardiac Surgery

R

Royal Brompton & Harefield NHS Foundation Trust

Status and phase

Terminated
Phase 2

Conditions

Cardiopulmonary Bypass
Blood Platelet Disorders
Cardiac Surgical Procedures
Blood Coagulation Disorders

Treatments

Procedure: Platelet rich plasma sequestration

Study type

Interventional

Funder types

Other

Identifiers

NCT00359398
2006IC003B

Details and patient eligibility

About

Excessive bleeding is common after cardiac surgery. This may result in patients receiving a blood transfusion or suffering the life-threatening complication of cardiac tamponade. Tamponade is when excessive bleeding compresses the heart and prevents it from pumping properly. A major reason for the bleeding is the damage done to platelets by the cardiopulmonary bypass (CPB) machine. Often patients receive platelets and plasma from blood donors to try to reduce the bleeding post-operatively. The investigators plan to take platelets and plasma from patients before they are damaged. They would then return these 'undamaged' sequestered platelets to the patients after the bypass machine is no longer needed. Therefore, the investigators' primary question is whether platelet sequestration would reduce the bleeding problems that occur following cardiac surgery. They will evaluate bleeding problems using thromboelastography, which provides a comprehensive assessment of both how blood clots form and their strength. If sequestration reduces bleeding problems following cardiac surgery then it may reduce the chance of patients receiving blood products from donors. Although donated blood is thoroughly tested, its use does expose patients to the risk of transfusion errors, blood borne infections and reactions. Avoiding its use would be very desirable.

Full description

Hypothesis:

Patients undergoing repeat median sternotomy or requiring prolonged cardiopulmonary bypass (CPB) often develop a coagulopathy at the end of surgery. We propose sequestering plasma and platelets from these patients prior to CPB and thus prior to the dilution and platelet damage that occurs with CPB. We hypothesise that if these plasma and platelets are stored properly during CPB, and administered at the end of the operation, they will reduce any coagulopathy and the associated bleeding.

Study population:

Patients undergoing repeat median sternotomy, mitral valve repair, double valve operations, aortic surgery, or combined valve and coronary artery surgery.

Exclusion criteria will include those with anaemia, thrombocytopenia, unstable angina, anti-platelet therapy within the previous seven days, known or symptomatic cerebrovascular disease, known disorders of haemostasis, aprotinin sensitivity and pregnancy.

Intervention:

Patients will be randomised by a closed envelope technique to receive platelet/plasma sequestration or not. Patients randomised to undergo sequestration will have 14 mL/Kg blood taken. The blood will be separated into red cells and platelets/plasma. Anaemia would be prevented by returning the processed red blood cells to the patient. We will store platelets at 20-24°C/room temperature on a platelet rocker according to guidelines from the National Blood Service.

Assessment of coagulation:

Patients' coagulation status will be evaluated before and after surgery. Four methods will be employed to comprehensively assess the coagulation system: platelet counts and conventional clotting studies; heparin levels; thromboelastography; and platelet function analyser.

Enrollment

30 patients

Sex

All

Ages

16+ years old

Volunteers

No Healthy Volunteers

Inclusion criteria

Adult patients undergoing cardiac surgery that involves:

  • Repeat median sternotomy
  • Mitral valve repair
  • Double valve operations
  • Combined valve and coronary surgery
  • Anticipated prolonged cardiopulmonary bypass

Exclusion criteria

  • Pre-operative anaemia
  • Pre-operative thrombocytopenia
  • Unstable angina
  • Anti-platelet therapy (e.g. aspirin, clopidogrel) within the previous 7 days
  • Known or symptomatic cerebrovascular disease
  • Known disorders of haemostasis
  • Aprotinin sensitivity
  • Pregnancy.

Trial design

Primary purpose

Treatment

Allocation

Randomized

Interventional model

Parallel Assignment

Masking

Single Blind

30 participants in 2 patient groups

Platelet sequestration
Experimental group
Description:
Sequestration of platelet rich plasma before cardiopulmonary bypass
Treatment:
Procedure: Platelet rich plasma sequestration
Standard care
No Intervention group
Description:
No platelet rich plasma sequestration undertaken before cardiopulmonary bypass (usual practice)

Trial contacts and locations

1

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Data sourced from clinicaltrials.gov

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