Serum and Semen Vitamin D Level With Semen Parameters

Assiut University

Status: Unknown

Conditions: Vitamin D Deficiency

Treatments: Diagnostic Test: ELISA to evaluate serum and seminal vitamin D level

Study type: Observational

Funder types: Other

Identifiers

NCT03477136

SSVDSS

Details and patient eligibility

About

vitamin D may have multiple functions, one of them is modulating reproductive processes and the reproductive role of vitamin D is highlighted by expression of the vitamin D receptors in testis, male reproductive tract and sperms

Full description

Semen analysis is the corner stone of infertility evaluation as it provides information on the functional status of the seminiferous tubules, epididymis and accessory sex glands. The methods on how the semen should be evaluated are provided by the World Health Organization Vitamin D is synthesized mainly in the skin, where ultraviolet ray B radiation converts 7-dehydrocholesterol to Vitamin D3. Then, Vitamin D3 undergoes two hydroxylation processes first in the liver and second in the kidney Serum 25(OH)D is the major circulating metabolite of vitamin D and reflects vitamin D inputs from cutaneous synthesis and dietary intake. The serum 25(OH)D level is the standard clinical measure of vitamin D status Vitamin D deficiency has been linked to various health disorders including boneheallth, cardiovascular, infectious, oncologic, musculoskeletal, neuropsychologic and reproductive disorders, as well as to overall mortality

Enrollment: 150 estimated patients

Sex: Male

Ages: 20 to 50 years old

Volunteers: No Healthy Volunteers

Inclusion and exclusion criteria

Inclusion Criteria

• -males with different semen parameter finding
• Age: 20-50 years

Exclusion Criteria:

1. Varicocele.
2. Genital tract infections.
3. Primary and secondary hypogonadism
4. Hormonal therapy.
5. Calcium and vitamin D supplement.
6. Hepatic or renal failure
7. Diseases or drugs affecting vitamin D absorption -

Trial design

150 participants in 5 patient groups

First group: semen parameter showing normospermic

Description:

* group will include 30 male * Semen samples will be collected from all patients Semen analysis will be examined after 2-5 days of sexual abstinence based on World Health Organization (WHO) (2010) * blood and semen sample will be collected from all males to evaluate vitamin D level by ELISA .

Treatment:

Diagnostic Test: ELISA to evaluate serum and seminal vitamin D level

second group: semen parameter asthenozoospermic

Description:

* group will include 30 male * Semen samples will be collected from all patients Semen analysis will be examined after 2-5 days of sexual abstinence based on World Health Organization (WHO) (2010) * blood and semen sample will be collected from all males to evaluate vitamin D level by ELISA .

Treatment:

Diagnostic Test: ELISA to evaluate serum and seminal vitamin D level

Third group: semen parameter oligozoospermic

Description:

* group will include 30 male * Semen samples will be collected from all patients Semen analysis will be examined after 2-5 days of sexual abstinence based on World Health Organization (WHO) (2010) * blood and semen sample will be collected from all males to evaluate vitamin D level by ELISA .

Treatment:

Diagnostic Test: ELISA to evaluate serum and seminal vitamin D level

Forth groupsemen parameter: astheno-teratozoospermic,

Description:

* group will include 30 male * Semen samples will be collected from all patients Semen analysis will be examined after 2-5 days of sexual abstinence based on World Health Organization (WHO) (2010) * blood and semen sample will be collected from all males to evaluate vitamin D level by ELISA .

Treatment:

Diagnostic Test: ELISA to evaluate serum and seminal vitamin D level

Fifth group semen parameter: oligo asthenoteratozoospermia

Description:

* group will include 30 male * Semen samples will be collected from all patients Semen analysis will be examined after 2-5 days of sexual abstinence based on World Health Organization (WHO) (2010) * blood and semen sample will be collected from all males to evaluate vitamin D level by ELISA .

Treatment:

Diagnostic Test: ELISA to evaluate serum and seminal vitamin D level