Serum Proteomics Analysis for Sepsis

Chinese PLA General Hospital (301 Hospital)

Status: Unknown

Conditions: Death; SIRS; Severe Sepsis; Sepsis

Study type: Observational

Funder types: Other

Identifiers

NCT01493466

20111013-007(2)

2009BAI86B03 (Other Grant/Funding Number)

Details and patient eligibility

About

Serum proteomics is a very useful tool to identify various disease. The purpose of the present study was to find differential proteins among patient with normal, SIRS, sepsis, severe sepsis, death and to screen potential biomarkers for their dynamic changes. Serum proteins were identified by iTRAQ labeling and LC-MS/MS. The bioinformatics analysis was performed with the Mascot software and the International Protein Index (IPI) and the Gene Ontology (GO) Database and KEGG pathway Database. The differentially expressed proteins were verified by Western blot by another sample collected from clinical.

Enrollment: 100 estimated patients

Sex: All

Ages: 18+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Male and female aged 18 years old and over;
• clinically confirmed infection;
• fulfilled at least two criteria of systemic inflammatory response syndrome
• (a) core temperature higher than 38 °C or lower than 36 °C
• (b)respiratory rate above 20/min, or PCO2 below 32 mmHg
• (c) pulse rate above 90/min, and
• (d) white blood cell count greater than 12,000/μl or lower than < 4,000/μl or less than 10% of bands.

Exclusion criteria

• younger than 18 years of age;
• acquired immunodeficiency syndrome;
• reduced polymorphonuclear granulocyte counts (< 500 μL-1);
• died within 24h after admission into the ICU, or refused to participate in the study, or declined treatment during the period of observation.

Trial design

100 participants in 5 patient groups

Normal

Description:

normal person under physical examination

SIRS

Description:

1. temperature \>38 ℃ or \<36℃; 2. pulse rate\>90 beats/min; 3. ventilatory rate\>20 breaths/min or hyperventilation with partial pressure of arterial carbon dioxide (PaCO2)\<32mmHg; 4. white blood cell count\>12,000μL-1 or \<4000μL-1 or \>10% immature cells

spesis

Description:

sepsis is defined as SIRS plus confirmed infection.

severe sepsis

Description:

1. severe sepsis: sepsis associated with organ dysfunction, hypoperfusion, or hypotension; 2. septic shock: sepsis with arterial hypotension, despite adequate fluid resuscitation.

death

Description:

sepsis patients within 48 hours before death